Screening Of Selected Fungal Collection For Resistance

• Main Author: Fronia Fernanda, Dinrark
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2018
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/35157/1/Screening%20Of%20Selected%20Fungal%20Collection%20For%20Resistance%2824pgs%29.pdf; http://ir.unimas.my/id/eprint/35157/4/Fronia%20Femanda%20Dinrark%20ft.pdf; http://ir.unimas.my/id/eprint/35157/

Glyphosate is a non-selective broad-spectrum herbicide that commonly been used in the agricultural system to controls the weed populations but also affects microbial populations especially fungi in soil. Plants that were infected by plant pathogenic fungi may affect the crop quality and yield quantity. However, the problem is not all types of fungi are susceptible towards glyphosate because certain types of fungi have different physiological response towards glyphosate. For instance, there are some fungi are resistance towards glyphosate due to the utilization of glyphosate as their food sources and some fungi that are susceptible towards glyphosate due to the interference in fungi shikimate pathway. The significance of this study was to study the physiological response of fungi towards glyphosate. The response of fungi towards glyphosate were monitored by applying a range of glyphosate concentrations (4 000, 8 000, 16 000, and 20 000 ppm) to potato dextrose agar (PDA) medium and following changes in physiological response of ten fungal species for 11 consecutive days in regard to their susceptibility, tolerance and resistance towards glyphosate. The screening of the treatment has been done by in vitro and the interaction between glyphosate concentration and the radial growth often fungal species. Results showed that susceptible fungi were Aspergillus aculeatus, Colletotrichum gloeosporioides, Marasmius cladophyllus UMAS MS8, and Neurospora crassa, while the tolerance fungi were Aspergil/us versicolor, Colletotrichum gloeosporioides, Cunninghamella bainieri, Penicillium citrinum, and Penicillium pinophilum, and the only resistance fungi was Aspergillus flavus. The fungi also had been identified by morphological and molecular characterization for each of the fungal species.