Screening of Antifungal Activity of Premna cordifolia leaves extract
Premna cordifolia (Roxb.) is a Malaysian traditional vegetable from genus Premna of the family Lamiaceae. It is known as 'bebuas' or 'daun singkil' in Malay. The aims of this study are to extract the, P. cordifolia leaves with methanol and dichloromethane extractions by using m...
Saved in:
Main Author: | |
---|---|
Format: | Final Year Project Report |
Language: | English |
Published: |
Universiti Malaysia Sarawak (UNIMAS)
2018
|
Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/35150/2/Noor%20Wahidah.pdf http://ir.unimas.my/id/eprint/35150/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Premna cordifolia (Roxb.) is a Malaysian traditional vegetable from genus Premna of the family Lamiaceae. It is
known as 'bebuas' or 'daun singkil' in Malay. The aims of this study are to extract the, P. cordifolia leaves with
methanol and dichloromethane extractions by using maceration technique and to screen the antifungal activity of
different solvents of leaf extracts at various concentrations against Aspergillus flavus, Aspergillus niger and
Fusarium solani. The leaf extracts were diluted into different concentration ( I 00 mg/mL, 50 mg/mL and 25
mg/mL) in 5% dimethyl sulfoxide (DMSO). The different concentration of different leaf extracts solvent were
spread onto Potato Dextrose Agar (PDA) plates respectively using poisoned food technique so that the growth of
the fungi can be screened. The result shows that 50 mg/mL of methanol extract shows the highest inhibition
percentage (68.38%) against F. solani, followed by 50 mg/mL and 25 mg/mL of methanol extract against A.
flavus with 64.71% and 61.76%, respectively. The methanol extracts of P. cordifolia leaves have greater
inhibition effect against A. flavus, A. niger and F. solani compared to dichloromethane extracts. The methanol
extracts with 50 mg/mL and 25 mg/mL displayed the highest inhibition effect. Dichloromethane extracts with
concentration of 100 mg/ml and 50 mg/ml show moderate inhibition effect against the tested fungi. This study
shows that P. cordifolia leaves does not only functioned as medicinal herbs whilst it is also effective against fungi
inhibitory activity. The antifungal compounds extracted from the leaves displayed its potential in inhibiting the
tested fungi from growing may lead to the development of alternative treatment for fungi infection. |
---|