Isolation and Enumeration of Vibrio cholerae From Fruit Juice
, cholerae is a microorganism that are water-borne and could be found in any water body, including water systems in any buildings. This study was conducted in order to detect the presence of W. cholerae in fruit juice from food premises around Kota Samarahan by isolating and enumerating the V cho!...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak (UNIMAS)
2018
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/35149/2/Nadzirah.pdf http://ir.unimas.my/id/eprint/35149/ |
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| Summary: | , cholerae is a microorganism that are water-borne and could be found in any water body, including water
systems in any buildings. This study was conducted in order to detect the presence of W. cholerae in fruit
juice from food premises around Kota Samarahan by isolating and enumerating the V cho!erae from fruit
juice that obtained from food premises around Kota Samarahan. The method that was applied in this research
are Most Probable Number test and Polymerase Chain Reaction. Serial dilution used in Most Probable
Number test are 10,10° and 10°. Most of the isolates have the MPN/g value that in range of <3.0 to 53
MPN/g. Total sample(n=30) show no band on the PCR assay with ctxA gene as positive control. It can be
conclude that the fruit juices around Kota Samarahan able to be contaminated by V. cho!erae due to several
reason. |
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