Genetic Structure of Metaphrynella sundana in Sarawak
Information of genetic structure of Metaphrynella sundana (Anura: Microhylidae) in Sarawak is lacking. Cytochrome b (cyt b) was used to infer this study in Batang Ai, Matang and Jagoi populations. A total of 582 bp from 800 bp of cyt b gene from six samples were extracted using Cetyl trimethylammoni...
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Format: | Final Year Project Report |
Language: | English |
Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/34303/1/Genetic%20Structure%20of%20Metaphrynella%20sundana%20in%20Sarawak%20%28fulltext%29.pdf http://ir.unimas.my/id/eprint/34303/ |
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Summary: | Information of genetic structure of Metaphrynella sundana (Anura: Microhylidae) in Sarawak is lacking. Cytochrome b (cyt b) was used to infer this study in Batang Ai, Matang and Jagoi populations. A total of 582 bp from 800 bp of cyt b gene from six samples were extracted using Cetyl trimethylammonium bromide (CTAB) protocol and it was being amplified using Polymerase Chain Reaction (PCR) approach. Genetic
structuring of M. sundana within all populations determined based on haplotype structure. No major clades of western and eastern part of Sarawak was observed with no sharing of
haplotype between populations, hence suggesting M. sundana as a more recent lineage. High migration events (Nm) and low population subdivision (Fsc) values indicated that the
genetic between populations are homogenous in spite of historical association among western and eastern population of M. sundana in Sarawak, thus suggesting that this species
dispersed throughout Sarawak. Population of M. sundana was also said undergone panmixia and the population is still expanding which supported by P-values of Fu's Fs test of neutrality. |
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