The effect of PH on sodium dodecyl sulphate towards zebrafish larvae development

• Main Author: Muhammad Khairulanam, Zakaria
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
• Subjects: Q Science (General); QD Chemistry
• Online Access: http://ir.unimas.my/id/eprint/32487/2/The%20effect%20of%20PH%20on%20sodium%20dodecyl%20sulphate%20towards%20zebrafish%20larvae%20development%20%28fulltext%29.pdf; http://ir.unimas.my/id/eprint/32487/

SDS is a widely used as chemical in industrial cleaner and in personal care such as shampoo, toothpaste and soap. The used of SDS as detegent may leads to negative effects towards aquatic ecosystem. Since water such as pH Level may vary between different aquatic ecosystems, it is therefore beneficial to study this water parameter affect towards SDS toxicity. Therefore, the main objective in this research is to study the effect of pH on SDS toxicity towards zebrafish larvae temporal development. Besides that, this study is conducted to observe any phenotypic deformities caused by SDS towards zebrafish larvae development under different pH conditions. The zebrafish (30 eggs per treatments and controls) is subjected to SDS (0.000004%) under different pH conditions which are pH 5.86 and pH 6.95. In this experiment, hatching rate (p<0.218), free swim rate (p<0.56) and survival rate (p<O. 168) as not affected by different pH conditions. Besides that, there were no significant differences on the rate of phenotypic deformities (pericardial edema, yolk sac edema and vertebral bent) that was caused by the SDS in different pH condition and also that was caused by altering the pH alone. However, the pericardial edema and yolk sac edema only appears when SDS were presents, albeit at a very low percentage that leads to the insignificant difference from statistical analysis. In conclusion, the effect of altering the pH itself (pH 5.86 and pH 6.95) has resulted in profound effects that could mask the effect of SDS on zebrafish larvae development.