Breeding System and Phytochemical analysis of Orthosiphon aristatus Blume (Miq.)
Orthosiphon aristatus Blume (Miq.) is a perennial herb under the mint family, Lamiaceae. The study on 0. aristatus was conducted with several aims to accomplish which include (I) to study the breeding system of O. aristatus through artificial pollination, (2) to determine the flower developmental...
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Format: | Final Year Project Report |
Language: | English |
Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/31985/1/Breeding%20System%20and%20Phytochemical%20analysis%20of%20Orthosiphon%20aristatus%20Blume%20%28Miq.%29%28fulltext%29.pdf http://ir.unimas.my/id/eprint/31985/ |
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Summary: | Orthosiphon aristatus Blume (Miq.) is a perennial herb under the mint family, Lamiaceae. The study on 0.
aristatus was conducted with several aims to accomplish which include (I) to study the breeding system of
O. aristatus through artificial pollination, (2) to determine the flower developmental at which the pollen is
most viable, (3) to investigate the potential of flavonoids as phytochemical markers, and (4) to determine
whether there is any significant effect of different drying conditions on the flavonoid profiles of 0. aristatus.
In artificial pollination tests, two tests conducted which were self-pollination and cross pollination. All
artificial pollination tests were considered as successful if seeds were developed. Pollen viability test were
conducted using in vitro germination method and the pollens were collected from three different
developmental stages; pre-anthesis, anthesis and post-anthesis. Whilst, for the phytochemical marker analysis
of flavonoids, two dimensional paper chromatography (PC) was utilised as the separation technique.
Retention factor (R) value was calculated for each compound observed and the flavonoid profiles of OAV-I
and OAV-2 were compared. Lastly, the different drying conditions were imposed on the 0. aristatus samples
to see if there is any significant effect on the flavonoids content of the leaf samples. From the study
conducted, 0. aristatus was suggested to be a pre-dominantly self-pollinated species with the support of
pollen viability test that indicated viable pollen can be observed during anthesis stage. The flavonoid profiles
of OAV-I and OAV-2 showed polymorphism which can be used as marker in breeding program. Drying
quality test revealed that temperature plays important factor in the flavonoid contents of O. aristatus leaf
samples. |
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