New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi
Background Plasmodium knowlesi is a significant cause of human malaria in Sarawak, Malaysian Borneo. Only one study has been previously undertaken in Sarawak to identify vectors of P. knowlesi, where Anopheles latens was incriminated as the vector in Kapit, central Sarawak. A study was therefore un...
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Springer Nature
2020
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Online Access: | http://ir.unimas.my/id/eprint/31850/1/New%20vectors%20in%20northern%20Sarawak%2C%20Malaysian%20Borneo%2C%20for%20the%20zoonotic%20malaria%20parasite%2C%20Plasmodium%20knowlesi.pdf http://ir.unimas.my/id/eprint/31850/ https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04345-2?utm_source=other&utm_medium=other&utm_content=null&utm_campaign=BSCN_2_DD01_CN_bmcso_article_paid_XMOL |
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my.unimas.ir.318502020-09-18T08:37:53Z http://ir.unimas.my/id/eprint/31850/ New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi Ang Xin De, J. Kadir, Khamisah Abdul Mohamad, D.S. Matusop, A. Divis, Paul Cliff Simon Yaman, Khatijah Singh, Balbir Q Science (General) QR Microbiology RA0421 Public health. Hygiene. Preventive Medicine Background Plasmodium knowlesi is a significant cause of human malaria in Sarawak, Malaysian Borneo. Only one study has been previously undertaken in Sarawak to identify vectors of P. knowlesi, where Anopheles latens was incriminated as the vector in Kapit, central Sarawak. A study was therefore undertaken to identify malaria vectors in a different location in Sarawak. Methods Mosquitoes found landing on humans and resting on leaves over a 5-day period at two sites in the Lawas District of northern Sarawak were collected and identified. DNA samples extracted from salivary glands of Anopheles mosquitoes were subjected to nested PCR malaria-detection assays. The small subunit ribosomal RNA (SSU rRNA) gene of Plasmodium was sequenced, and the internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of the mosquitoes were sequenced from the Plasmodium-positive samples for phylogenetic analysis. Results Totals of 65 anophelines and 127 culicines were collected. By PCR, 6 An. balabacensis and 5 An. donaldi were found to have single P. knowlesi infections while 3 other An. balabacensis had either single, double or triple infections with P. inui, P. fieldi, P. cynomolgi and P. knowlesi. Phylogenetic analysis of the Plasmodium SSU rRNA gene confirmed 3 An. donaldi and 3 An. balabacensis with single P. knowlesi infections, while 3 other An. balabacensis had two or more Plasmodium species of P. inui, P. knowlesi, P. cynomolgi and some species of Plasmodium that could not be conclusively identified. Phylogenies inferred from the ITS2 and/or cox1 sequences of An. balabacensis and An. donaldi indicate that they are genetically indistinguishable from An. balabacensis and An. donaldi, respectively, found in Sabah, Malaysian Borneo. Conclusions Previously An. latens was identified as the vector for P. knowlesi in Kapit, central Sarawak, Malaysian Borneo, and now An. balabacensis and An. donaldi have been incriminated as vectors for zoonotic malaria in Lawas, northern Sarawak. Springer Nature 2020-09-15 Article PeerReviewed text en http://ir.unimas.my/id/eprint/31850/1/New%20vectors%20in%20northern%20Sarawak%2C%20Malaysian%20Borneo%2C%20for%20the%20zoonotic%20malaria%20parasite%2C%20Plasmodium%20knowlesi.pdf Ang Xin De, J. and Kadir, Khamisah Abdul and Mohamad, D.S. and Matusop, A. and Divis, Paul Cliff Simon and Yaman, Khatijah and Singh, Balbir (2020) New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi. Parasites & Vectors, 13 (472). pp. 1-13. ISSN 1756-3305 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04345-2?utm_source=other&utm_medium=other&utm_content=null&utm_campaign=BSCN_2_DD01_CN_bmcso_article_paid_XMOL 10.1186/s13071-020-04345-2 |
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Q Science (General) QR Microbiology RA0421 Public health. Hygiene. Preventive Medicine Ang Xin De, J. Kadir, Khamisah Abdul Mohamad, D.S. Matusop, A. Divis, Paul Cliff Simon Yaman, Khatijah Singh, Balbir New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi |
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Background
Plasmodium knowlesi is a significant cause of human malaria in Sarawak, Malaysian Borneo. Only one study has been previously undertaken in Sarawak to identify vectors of P. knowlesi, where Anopheles latens was incriminated as the vector in Kapit, central Sarawak. A study was therefore undertaken to identify malaria vectors in a different location in Sarawak.
Methods
Mosquitoes found landing on humans and resting on leaves over a 5-day period at two sites in the Lawas District of northern Sarawak were collected and identified. DNA samples extracted from salivary glands of Anopheles mosquitoes were subjected to nested PCR malaria-detection assays. The small subunit ribosomal RNA (SSU rRNA) gene of Plasmodium was sequenced, and the internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (cox1) gene of the mosquitoes were sequenced from the Plasmodium-positive samples for phylogenetic analysis.
Results
Totals of 65 anophelines and 127 culicines were collected. By PCR, 6 An. balabacensis and 5 An. donaldi were found to have single P. knowlesi infections while 3 other An. balabacensis had either single, double or triple infections with P. inui, P. fieldi, P. cynomolgi and P. knowlesi. Phylogenetic analysis of the Plasmodium SSU rRNA gene confirmed 3 An. donaldi and 3 An. balabacensis with single P. knowlesi infections, while 3 other An. balabacensis had two or more Plasmodium species of P. inui, P. knowlesi, P. cynomolgi and some species of Plasmodium that could not be conclusively identified. Phylogenies inferred from the ITS2 and/or cox1 sequences of An. balabacensis and An. donaldi indicate that they are genetically indistinguishable from An. balabacensis and An. donaldi, respectively, found in Sabah, Malaysian Borneo.
Conclusions
Previously An. latens was identified as the vector for P. knowlesi in Kapit, central Sarawak, Malaysian Borneo, and now An. balabacensis and An. donaldi have been incriminated as vectors for zoonotic malaria in Lawas, northern Sarawak. |
format |
Article |
author |
Ang Xin De, J. Kadir, Khamisah Abdul Mohamad, D.S. Matusop, A. Divis, Paul Cliff Simon Yaman, Khatijah Singh, Balbir |
author_facet |
Ang Xin De, J. Kadir, Khamisah Abdul Mohamad, D.S. Matusop, A. Divis, Paul Cliff Simon Yaman, Khatijah Singh, Balbir |
author_sort |
Ang Xin De, J. |
title |
New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi |
title_short |
New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi |
title_full |
New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi |
title_fullStr |
New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi |
title_full_unstemmed |
New vectors in northern Sarawak, Malaysian Borneo, for the zoonotic malaria parasite, Plasmodium knowlesi |
title_sort |
new vectors in northern sarawak, malaysian borneo, for the zoonotic malaria parasite, plasmodium knowlesi |
publisher |
Springer Nature |
publishDate |
2020 |
url |
http://ir.unimas.my/id/eprint/31850/1/New%20vectors%20in%20northern%20Sarawak%2C%20Malaysian%20Borneo%2C%20for%20the%20zoonotic%20malaria%20parasite%2C%20Plasmodium%20knowlesi.pdf http://ir.unimas.my/id/eprint/31850/ https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04345-2?utm_source=other&utm_medium=other&utm_content=null&utm_campaign=BSCN_2_DD01_CN_bmcso_article_paid_XMOL |
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1678596417163100160 |
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13.15806 |