TLC separation of L-tryptophan using microemulsion mobile phase and its spectrophotometric determination
The TLC system comprising silica gel as the stationary phase, and water- in –oil microemul sion (SDS+water+heptane+n-pentanol; (160 g + 8 mL + 16 mL + 25 mL) as the mobile phase, has been identified as the most favourable system for selective separation of L-tryptophan from other amino acids in the...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | E-Article |
| Language: | English |
| Published: |
NISCAIR-CSIR, India
2006
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/29697/1/TLC%20separation%20of%20L-tryptophan%20using%20microemulsion%20mobile%20phase%20-%20Copy.pdf http://ir.unimas.my/id/eprint/29697/ http://nopr.niscair.res.in/handle/123456789/20019 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The TLC system comprising silica gel as the stationary phase, and water- in –oil microemul sion (SDS+water+heptane+n-pentanol; (160 g + 8 mL + 16 mL + 25 mL) as the mobile phase, has been identified as the most favourable system for selective separation of L-tryptophan from other amino acids in the presence of metal cation impurities. The proposed method is applicable to the identification and separation of L-tryptophan from drug samples such as Astymin -m (Forte), Astymin (liquid) and Alamin (Forte). The separation up to 10μg of L-tryptophan from milligram quantities (up to 0.77 mg) of other amino acids has also been realized. The quantitative estimation of L-tryptophan by spectrophotometry (λmax 570 nm) after separation from other amino acids has been done. The recovery of tryptophan is around 98% and its limit of detection on silica gel layer is 0.13 μg. |
|---|