Formulation development of crude oil bioremediation by indigeneous bacteria isolated from oily sludge

• Main Author: Nur Hafizah, Binti Azizan
• Format: Thesis
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2009
• Subjects: TP Chemical technology
• Online Access: http://ir.unimas.my/id/eprint/28271/1/Nur%20Hafizah%20Azizan%20ft.pdf; http://ir.unimas.my/id/eprint/28271/

Enrichment culture technique lead to the discovery of three presumptive TPHdegrading bacteria. Identification and characterization tests using morphological, biochemical and molecular techniques revealed that the bacteria isolates were Pseudomonas aeruginosa (LIMAS 1 PF), Serratia marcescens (UMAS2SF) and Klebsiella spp. (UMAS3KF) with 99%, 98% and 84% nucleotide similarity, respectively. These isolates were able to use crude oil as sole carbon and energy source for their growth since they were able to survive in Minimal Salt media supplemented with 1% (v/v) crude oil. Growth study showed that isolates produced the highest cell counts on the third or fourth day by' 108 - 1011 CFU/ml. Six artificial consortia have been produced from the growth study. Gas chromatography analysis showed that all isolates had the ability to degrade aliphatic hydrocarbon with 100% degradation of nC19 - C24 were recorded. Among the isolates, UMAS2SF was the best and fastest n-alkane degrader as they degraded between 55 - 90% of n-C14 - C, 8 in 14 days. This was followed by UMASIPF and UMAS3KF with 11 - 82% and 1.3% degradation, respectively. Enhancement study showed that plot with inoculum and NPK addition successfully enhanced n-alkane degradation. Plot A2: B3+NPK degraded n-alkane the fastest followed by plot treated by C+NPK, Al: B2, B+NPK and A2: B3. Result showed that UMAS IPF was the best PAHs degrader as most of the high molecular weight PAHs was degraded. In the enhancement study, the plot amended with A2: B3 showed the highest PAHs degradation, followed by plots Al: B2, A3: BI: C2 and Al: C3 that was assigned as the third, fourth and fifth best in mineralizing PAHs, respectively.