An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak

Infectious laryngotracheitis (I LT) is an acute respiratory disease aflecting chickens. The disease is caused by infectious laryngotracheitis virus (IL TV). The detection of ILTV by polymerase chain reaction (PCR) has not been reported in Sarawak as no major outbreaks have occurred. This study repor...

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Main Author: Shaiful Azhan, Bin Malik
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2007
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Online Access:http://ir.unimas.my/id/eprint/26881/2/Shaiful%20Azhan.pdf
http://ir.unimas.my/id/eprint/26881/
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spelling my.unimas.ir.268812024-03-06T08:04:02Z http://ir.unimas.my/id/eprint/26881/ An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak Shaiful Azhan, Bin Malik Q Science (General) QR Microbiology Infectious laryngotracheitis (I LT) is an acute respiratory disease aflecting chickens. The disease is caused by infectious laryngotracheitis virus (IL TV). The detection of ILTV by polymerase chain reaction (PCR) has not been reported in Sarawak as no major outbreaks have occurred. This study reports the application of PCR in an attempt to detect the presence of ILTV in village chickens in Kuching Division. The external observation and post-mortem examination had been performed on selected village chickens for finding the symptoms and signs that were related to I LTV. The total DN A had been extracted from 46 serum samples, 46 blood samples and 48 laryngeal tissue samples by henollchloroformfisoamylalcohol (PCI) extraction method. No symptoms of ILTV on village chickens and no signs of IL TV in trachea and laryngeal tissue had been found. The extracted DNA was amplified using ILTU2 and ILTL2 primers via PCR. The PCR analysis shows no live and dormant ILTV in the village chickens in Kuching Division. The positive control that was used in this study is modified attenuated ILTV vaccine. A band pattern from the positive control could be observed after visualization under Ultraviolet (UV) transilluminator. Further similar studies can be performed in other divisions for determining the geographical distribution o f J LTV in village chickens in Sarawak. Universiti Malaysia Sarawak (UNIMAS) 2007 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/26881/2/Shaiful%20Azhan.pdf Shaiful Azhan, Bin Malik (2007) An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Shaiful Azhan, Bin Malik
An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak
description Infectious laryngotracheitis (I LT) is an acute respiratory disease aflecting chickens. The disease is caused by infectious laryngotracheitis virus (IL TV). The detection of ILTV by polymerase chain reaction (PCR) has not been reported in Sarawak as no major outbreaks have occurred. This study reports the application of PCR in an attempt to detect the presence of ILTV in village chickens in Kuching Division. The external observation and post-mortem examination had been performed on selected village chickens for finding the symptoms and signs that were related to I LTV. The total DN A had been extracted from 46 serum samples, 46 blood samples and 48 laryngeal tissue samples by henollchloroformfisoamylalcohol (PCI) extraction method. No symptoms of ILTV on village chickens and no signs of IL TV in trachea and laryngeal tissue had been found. The extracted DNA was amplified using ILTU2 and ILTL2 primers via PCR. The PCR analysis shows no live and dormant ILTV in the village chickens in Kuching Division. The positive control that was used in this study is modified attenuated ILTV vaccine. A band pattern from the positive control could be observed after visualization under Ultraviolet (UV) transilluminator. Further similar studies can be performed in other divisions for determining the geographical distribution o f J LTV in village chickens in Sarawak.
format Final Year Project Report
author Shaiful Azhan, Bin Malik
author_facet Shaiful Azhan, Bin Malik
author_sort Shaiful Azhan, Bin Malik
title An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak
title_short An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak
title_full An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak
title_fullStr An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak
title_full_unstemmed An attempt to detect infectious Laryngotracheitis virus in village chickens in Kuching division, Sarawak
title_sort attempt to detect infectious laryngotracheitis virus in village chickens in kuching division, sarawak
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2007
url http://ir.unimas.my/id/eprint/26881/2/Shaiful%20Azhan.pdf
http://ir.unimas.my/id/eprint/26881/
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score 13.211869