Root Rot Disease and Rhizosphere Fungi of Acacia mangium in Sampadi Forest Plantation

• Main Author: Ahmad Syairazie, Ibrahim Fikri
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2014
• Subjects: SB Plant culture
• Online Access: http://ir.unimas.my/id/eprint/24497/1/Ahmad%20Syairazie%20Bin%20Ibrahim%20Fikri%20ft.pdf; http://ir.unimas.my/id/eprint/24497/

Acacia mangium is one of the major fast-growing species used in plantation forestry programs throughout Southeast Asia. Plantations of the forest species are the resource for a large pulp industry in Malaysia, especially in Sarawak. There was a report indicating that the planted A. mangium in Sampadi Plantation was infected by several diseases. The aims of the present research were to identify root rot, its causal pathogen and rhizosphere fungi in Acacia Plantation. Infected and healthy roots and the rhizosphere soils were collected at two sampling sites in the plantation area. In general observation, based on symptoms of root rot disease that were shown on the above plant part, the root rot were common in the plantation area. The isolation of microorganism from the samples were done using Malt Extract Agar (MEA), Potato Dextrose Agar (PDA), Soil Extract Agar (SEA) and Water Agar Agar (WA). The common microorganism isolated from infected root were varied depending on sampling site. They were of White sterile mycelia Sp 1., T. harzianum, and Bacteria. White sterile mycelia Sp 1. Was among the major microorganism isolates in both sampling site with percentage of occurrence more than 60.0%. White sterile mycelia Sp 1. were also isolated in the rhizosphere of infected root but not isolated in the rhizosphere of healthy root. Other microorganism that isolated from rhizosphere were Trichoderma harzianum, Paecilomyces lilacinus, Aspergillus flavus, Aspergillus niger and Bacteria. The identification of four isolates fungi were also supported by molecular work. They were P. janthinellum, C. quinqueseptatum, M. phaseolina and P. lilacinus. The growth of the fungi at the different temperature showed that most of the fungi grew significantly faster at (p=0.05) at temperature 30°C and mostly did not grow at 40°C except White sterile mycelia Sp 1., P. janthinellum and A. flavus. Effect of pH on growth was varied depending on the fungal isolates. In general, they can grew at wide range of pH.