Ex vitro grafting of papaya (carica papaya l.)
Papaya (Carica papaya L. ) is a polygamous fruit crop with three basic sex types i. e. male, female and bisexual. Papaya plants derived from seeds are not genetically true-to-type. Although vegetative propagation can be used to produce papaya with desired sex type but it is not efficient. Therefor...
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Main Author: | |
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Format: | Final Year Project Report |
Language: | English |
Published: |
Universiti Malaysia Sarawak (UNIMAS)
2010
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/24479/2/Liew%20Cai%20Foon.pdf http://ir.unimas.my/id/eprint/24479/ |
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Summary: | Papaya (Carica papaya L. ) is a polygamous fruit crop with three basic sex types i. e. male, female and
bisexual. Papaya plants derived from seeds are not genetically true-to-type. Although vegetative propagation
can be used to produce papaya with desired sex type but it is not efficient. Therefore, micropropagation by in
vitro techniques had been developed to mass propagate papaya. Since rooting efficiency of the tissue-culture
derived papaya shoots is still low, micrografting of papaya if successful can serve as an interim measure for
production of papaya planting material. The objective of this project is to develop a method for micrografting
of papaya ex vitro. Micrografting was done by using in vitro shoots as scions and papaya seedlings as
rootstocks. The seedlings were used as rootstocks when they reached 2-3 cm height in in vitro grafting. The
in vitro shoots had undergone three subcultures in modified DS media supplemented with 0.5 mg/L BAP and
0.2 mg/L NAA before transferred to media containing 0.5 mg/L GA3 for shoot elongation. The shoots were
used as scions when they reached 1-1.5 cm height. The grafting procedures were carried out both in vitro and
ex vitro. Two grafting methods i. e. wedge and saddle grafting had been tried. Wedge grafting was found to
be more successful than saddle grafting. |
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