Overexpression of recombinant domain III envelope protein of Zika virus
Aims: Zika virus (ZIKV) is a member of the Flaviviridae family and is transmitted to humans by mosquitoes. In humans, it causes disease known as Zika fever. The severity of the infection ranged from asymptomatic to mild disease and to infection associated with neurological disorders and congenital...
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Malaysian Society for Microbiology
2018
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my.unimas.ir.237862019-07-17T01:34:44Z http://ir.unimas.my/id/eprint/23786/ Overexpression of recombinant domain III envelope protein of Zika virus Sylvia, Empiang Andrew Easther, Manye Kennedy Magdline Sia, Henry Sum Q Science (General) QR180 Immunology QR355 Virology R Medicine (General) Aims: Zika virus (ZIKV) is a member of the Flaviviridae family and is transmitted to humans by mosquitoes. In humans, it causes disease known as Zika fever. The severity of the infection ranged from asymptomatic to mild disease and to infection associated with neurological disorders and congenital anomaly. The common symptoms are maculopapular rash, fever, arthralgia, myalgia, headache and conjunctivitis. The flavivirus genome consists of structural and nonstructural proteins. The envelope (E) glycoprotein is the major structural protein which is responsible for virus entry and represents a major target for neutralizing antibodies. The E protein consists of three distinct domains: domain I, domain II and domain III. The domain III (DIII) of the E protein has shown to be useful as antigen for flavivirus serologic diagnosis and immunization in animal model. Hence, the aim of this work is to express the DIII of E protein (EDIII) of ZIKV for immunoreactivity study. Methodology and results: The EDIII of ZIKV was cloned into pET SUMO cloning vector and transformed into Mach-T1 competent E. coli cells. Positive clone was selected, verified and transformed into BL21 (DE3) competent E. coli for protein expression. The expression of the recombinant protein was analysed on SDS-PAGE and western blot. The recombinant fusion protein of EDIII/SUMOHIS (rEDIII) was successfully expressed at a molecular weight of approximately 38.2 kDa. Conclusion, significance and impact of study: The expression of the protein was confirmed by detection with antihistidine and a flavivirus antiserum, HPR. Malaysian Society for Microbiology 2018-01-01 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/23786/3/Overexpression%20of%20recombinant%20domain%20%28abstract%29.pdf Sylvia, Empiang Andrew and Easther, Manye Kennedy and Magdline Sia, Henry Sum (2018) Overexpression of recombinant domain III envelope protein of Zika virus. Malaysian Journal of Microbiology, 14 (4). pp. 325-328. ISSN 1823-8262 http://mjm.usm.my/ http://dx.doi.org/10.21161/mjm.144185 |
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Q Science (General) QR180 Immunology QR355 Virology R Medicine (General) Sylvia, Empiang Andrew Easther, Manye Kennedy Magdline Sia, Henry Sum Overexpression of recombinant domain III envelope protein of Zika virus |
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Aims: Zika virus (ZIKV) is a member of the Flaviviridae family and is transmitted to humans by mosquitoes. In humans,
it causes disease known as Zika fever. The severity of the infection ranged from asymptomatic to mild disease and to
infection associated with neurological disorders and congenital anomaly. The common symptoms are maculopapular
rash, fever, arthralgia, myalgia, headache and conjunctivitis. The flavivirus genome consists of structural and nonstructural proteins. The envelope (E) glycoprotein is the major structural protein which is responsible for virus entry and
represents a major target for neutralizing antibodies. The E protein consists of three distinct domains: domain I, domain
II and domain III. The domain III (DIII) of the E protein has shown to be useful as antigen for flavivirus serologic
diagnosis and immunization in animal model. Hence, the aim of this work is to express the DIII of E protein (EDIII) of
ZIKV for immunoreactivity study.
Methodology and results: The EDIII of ZIKV was cloned into pET SUMO cloning vector and transformed into Mach-T1
competent E. coli cells. Positive clone was selected, verified and transformed into BL21 (DE3) competent E. coli for
protein expression. The expression of the recombinant protein was analysed on SDS-PAGE and western blot. The
recombinant fusion protein of EDIII/SUMOHIS (rEDIII) was successfully expressed at a molecular weight of
approximately 38.2 kDa.
Conclusion, significance and impact of study: The expression of the protein was confirmed by detection with antihistidine and a flavivirus antiserum, HPR. |
format |
E-Article |
author |
Sylvia, Empiang Andrew Easther, Manye Kennedy Magdline Sia, Henry Sum |
author_facet |
Sylvia, Empiang Andrew Easther, Manye Kennedy Magdline Sia, Henry Sum |
author_sort |
Sylvia, Empiang Andrew |
title |
Overexpression of recombinant domain III envelope protein of Zika virus |
title_short |
Overexpression of recombinant domain III envelope protein of Zika virus |
title_full |
Overexpression of recombinant domain III envelope protein of Zika virus |
title_fullStr |
Overexpression of recombinant domain III envelope protein of Zika virus |
title_full_unstemmed |
Overexpression of recombinant domain III envelope protein of Zika virus |
title_sort |
overexpression of recombinant domain iii envelope protein of zika virus |
publisher |
Malaysian Society for Microbiology |
publishDate |
2018 |
url |
http://ir.unimas.my/id/eprint/23786/3/Overexpression%20of%20recombinant%20domain%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/23786/ http://mjm.usm.my/ http://dx.doi.org/10.21161/mjm.144185 |
_version_ |
1644514141545693184 |
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13.159267 |