Modified bicinchoninic acid assay for accurate determination of variable length reducing sugars in carbohydrates

• Main Authors: Mohd Hasnain, Md Hussain, Zainab, Ngaini, N. F. M., Chong
• Format: Article
• Language: English
• Published: The International Food Research Journal (IFRJ) 2018
• Subjects: Q Science (General); QD Chemistry; QH301 Biology
• Online Access: http://ir.unimas.my/id/eprint/22537/1/Zainab.pdf; http://ir.unimas.my/id/eprint/22537/; http://www.ifrj.upm.edu.my/index.html

The accurate determination of reducing ends of malto-oligosaccharides is essential for calculating the enzyme activities of starch debranching enzymes. The suitability of the 3,5-Dinitrosalicylic acid (DNS) method, the Dygert method, and the Bicinchoninic acid (BCA) method for accurate determination...