Transformation of mungbeans via stratification of seeds

• Main Author: Nurul Liana, Binti Suardy
• Format: E-LPTA
• Language: English; English
• Published: unimas 2015
• Subjects: Q Science (General); SB Plant culture
• Online Access: http://ir.unimas.my/id/eprint/20841/1/Transformation%20of%20Mungbeans%2024pgs.pdf; http://ir.unimas.my/id/eprint/20841/2/Transformation%20of%20Mungbeans%20fulltext.pdf; http://ir.unimas.my/id/eprint/20841/

Mungbean (Vigna radiata (L.) Wilzeck) is one of the important crops used in daily life. Mungbean is rich in protein and easy to digest. Plant transformation can be conducted in several ways; either through direct DNA introduction or natural transfer mechanism. In this study, transformation was conducted using natural transfer mechanism via use of Agrobacterium tumefaciens. The Agrobacterium carrying the plamid pGSA1131, contains the antibiotic resistance towards chloramphenicol and a reporter gene, β-glucuronidase (GUS). For this work, the transformation was done via the use of stratified and non-stratified seeds. To facilitate the transformation, the seeds were exposed to vacuum infiltration and also the addition of acetosyringone. Analysis of putative transformants was done via extraction of plant DNA and detection of transgenes via polymerase chain reaction (PCR) technique. The PCR detecting the GUS reporter gene were undertaken and showed the presence of the gene in the putative transformants. It was also shown that stratification of the seeds facilitated the transfer and integration of transgenes.