Effects of castor oil on escherichia coli K011 growth and activities during fed-batch fermentation

• Main Author: Aizzul Amin, Aziz.
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2015
• Subjects: QD Chemistry; QE Geology; QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/19987/1/Effects%20of%20castor%20oil%20on%20Escherichia%20coli%20K011%20..%20%2824%20pages%29.pdf; http://ir.unimas.my/id/eprint/19987/8/Aizzul%20Amin%20Aziz%20ft.pdf; http://ir.unimas.my/id/eprint/19987/

The current depletion of fossil fuels, is causing excessive burdens in fuels supplied. This phenomenon has resulted in the rising price of fossil fuels globally. To resolve this issue, non-petroleum based fuels such as bioethanol is chosen as a primary alternative solution for fossil fuels energy. Traditionally, bioethanol is obtained from a process called fermentation. However during fermentation, ethanol is accumulated in the fermentation that effects growth and activity ofthe fermented bacteria. In this study, the production ofethanol was performed using Escherichia coli KO II , and castor oil will be introduced into the fermentation vessel as a selective separator to absorb and separate the ethanol from the broth. The variable sugar added in this experiment are glucose and xylose. During the study, the detection of bioethanol are analyzed using HighPerformance Liquid Chromatography (HPLC). The presence of castor oil sample, was determined by comparing with control sample. The experiment was conducted in glucose and xylose concentration level (5%, 10%, 20% and 30%) and the growth E. coli KO II will be monitor and calculate from 0 to 84 hr fermentation (4 days). According to the results obtained, the best concentration of glucose/xylose and effective for production of ethanol during fed-batch fermentation was 10% sugar concentration with highest ethanol production at 45.80 gil for castor oil and 46.53 gil for control broth respectively with the optimum glucose and xylose utilization. The result gained from experiment are expected to give a positive impact in the production ofethanol through fed-batch fermentation.