Molecular cloning of hypervariable regions (HVRII) from cellulose synthase gene in a tropical timber tree neolamarckia macrophylla

• Main Author: Nin., Audrie Chang Chia
• Format: E-LPTA
• Language: English; English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2014
• Subjects: QK Botany; QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/19986/1/Molecular%20cloning%20of%20hypervariable%20regions%20%28HVRII%29%20from%20cellulose%20...%20%2824%20pages%29.pdf; http://ir.unimas.my/id/eprint/19986/2/Molecular%20cloning%20of%20hypervariable%20regions%20%28HVRII%29%20from%20cellulose%20...%20%28fulltext%29.pdf; http://ir.unimas.my/id/eprint/19986/

Neolamarckia macrophylla which belongs to the family Rubiaceae is locally known as red kelampayan in Malaysia. It is valuable in wood, paper and pulp industries. It is important to conduct this research due to the special features of this species which are offast growing, resistant to pest and diseases and profitable in large scale production. Infonnation on cellulose synthase gene (CesA) especially the hypervariable region II (HVRII) involved in wood formation of red kelampayan is crucial in future applications. The aim of the study is to identify and sequence the hypervariable (HVRII) region of cellulose synthase gene. Total RNA was extracted from the developing xylem of red kelarnpayan. The cDNA of NmCesAHVRII was amplified using reverse transcription-polymerase chain reaction (RT -PCR) approach with forward degenerate primer, HVR2F (5'-TGYTATGTYCAGTTYCCWC-3') and reverse degenerate primer, HVR2R (5'GANCCRTARATCCAYCC- 3'). NmCesAIHVRII was successfully sequenced. NmCesAIHVRII was clustered to 1 distinct clade associated with secondary cell wall synthesis and development. This study will provide a rapid access to NmCesAHVRII sequences for further understanding of NmCesA protein's role in future application such as genetic improvement of red kelampayan. This also facilitates the future selection of trees with optimal cellulose content required for certain specific industries as well as synthesizing of artificial cellulose, hence increasing the economic development and growth in the country.