Dengue serotyping with a label-free DNA sensor
Dengue virus (DENV) is one of the most important mosquito-borne viruses in tropical and subtropical regions. Development of severe forms of dengue viral infection such as dengue fever (DF) and dengue hemorrhagic fever (DHF) has claimed many lives. The standard methods for detecting dengue virus are...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Royal Society of Chemistry
2018
|
Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/19679/1/Dengue.pdf http://ir.unimas.my/id/eprint/19679/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040187020&doi=10.1039%2fc7ay02131c&partnerID=40&md5=34f2578095014ecb7d55edf47c7c0a4b |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.unimas.ir.19679 |
---|---|
record_format |
eprints |
spelling |
my.unimas.ir.196792021-05-29T06:08:34Z http://ir.unimas.my/id/eprint/19679/ Dengue serotyping with a label-free DNA sensor S., K. Chan Y., S. Choong Perera, David Theam, Soon Lim QD Chemistry R Medicine (General) Dengue virus (DENV) is one of the most important mosquito-borne viruses in tropical and subtropical regions. Development of severe forms of dengue viral infection such as dengue fever (DF) and dengue hemorrhagic fever (DHF) has claimed many lives. The standard methods for detecting dengue virus are time consuming, laborious, and require skilful personnel. In this study, we propose a method whereby DENV RNA extracted from dengue infected mosquitoes was converted into DNA for probe hybridization to generate silver nanocluster strands that could be visualised under UV light. Label-free silver nanocluster based DNA sensors are able to provide strong fluorescence upon DNA hybridization. Highly specific DNA sequence detection is possible by taking advantage of the specificity of DNA hybridization kinetics. The proposed system is capable of detecting all four dengue DNA serotypes (DENV1-4) without any cross-reactivity. A single tube assay format showed better hybridisation efficiency with higher fluorescence intensity generated and a lower detection limit compared to a cocktail probe assay format. The method was able to detect as low as 100 nM of amplified double stranded dengue DNA targets using both single and cocktail probe assays. This provides an interesting alternative approach for multiplex DNA sensing utilizing DNA silver nanoclusters as a reporter system. © 2018 The Royal Society of Chemistry. Royal Society of Chemistry 2018 Article PeerReviewed text en http://ir.unimas.my/id/eprint/19679/1/Dengue.pdf S., K. Chan and Y., S. Choong and Perera, David and Theam, Soon Lim (2018) Dengue serotyping with a label-free DNA sensor. Analytical Methods, 10 (2). pp. 214-222. ISSN 1759-9660 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040187020&doi=10.1039%2fc7ay02131c&partnerID=40&md5=34f2578095014ecb7d55edf47c7c0a4b DOI: 10.1039/c7ay02131c |
institution |
Universiti Malaysia Sarawak |
building |
Centre for Academic Information Services (CAIS) |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Malaysia Sarawak |
content_source |
UNIMAS Institutional Repository |
url_provider |
http://ir.unimas.my/ |
language |
English |
topic |
QD Chemistry R Medicine (General) |
spellingShingle |
QD Chemistry R Medicine (General) S., K. Chan Y., S. Choong Perera, David Theam, Soon Lim Dengue serotyping with a label-free DNA sensor |
description |
Dengue virus (DENV) is one of the most important mosquito-borne viruses in tropical and subtropical regions. Development of severe forms of dengue viral infection such as dengue fever (DF) and dengue hemorrhagic fever (DHF) has claimed many lives. The standard methods for detecting dengue virus are time consuming, laborious, and require skilful personnel. In this study, we propose a method whereby DENV RNA extracted from dengue infected mosquitoes was converted into DNA for probe hybridization to generate silver nanocluster strands that could be visualised under UV light. Label-free silver nanocluster based DNA sensors are able to provide strong fluorescence upon DNA hybridization. Highly specific DNA sequence detection is possible by taking advantage of the specificity of DNA hybridization kinetics. The proposed system is capable of detecting all four dengue DNA serotypes (DENV1-4) without any cross-reactivity. A single tube assay format showed better hybridisation efficiency with higher fluorescence intensity generated and a lower detection limit compared to a cocktail probe assay format. The method was able to detect as low as 100 nM of amplified double stranded dengue DNA targets using both single and cocktail probe assays. This provides an interesting alternative approach for multiplex DNA sensing utilizing DNA silver nanoclusters as a reporter system. © 2018 The Royal Society of Chemistry. |
format |
Article |
author |
S., K. Chan Y., S. Choong Perera, David Theam, Soon Lim |
author_facet |
S., K. Chan Y., S. Choong Perera, David Theam, Soon Lim |
author_sort |
S., K. Chan |
title |
Dengue serotyping with a label-free DNA sensor |
title_short |
Dengue serotyping with a label-free DNA sensor |
title_full |
Dengue serotyping with a label-free DNA sensor |
title_fullStr |
Dengue serotyping with a label-free DNA sensor |
title_full_unstemmed |
Dengue serotyping with a label-free DNA sensor |
title_sort |
dengue serotyping with a label-free dna sensor |
publisher |
Royal Society of Chemistry |
publishDate |
2018 |
url |
http://ir.unimas.my/id/eprint/19679/1/Dengue.pdf http://ir.unimas.my/id/eprint/19679/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85040187020&doi=10.1039%2fc7ay02131c&partnerID=40&md5=34f2578095014ecb7d55edf47c7c0a4b |
_version_ |
1701166553469288448 |
score |
13.18916 |