Study of reproductive activity, maturity stages and tetrodotoxin (TTX) in yellow pufferfish, xenopterus naritus

• Main Author: Herman Hermawan, Bakr i
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2016
• Subjects: QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/18785/3/Herman.pdf; http://ir.unimas.my/id/eprint/18785/

This study was carried out in order to document the reproductive activity, maturity stages and tetrodotoxin (TTX) distribution in yellow pufferfish, Xenopterus naritus from Batang Saribas, Sarawak. The reproductive activity study shows the result of gonadosomativ index (GSI), hepatosomatic index (HSI) and fecundity, which both male and female individuals are found to be the highest in August (GSI: male-0.95%, female-12.73%; HSI:male-3.44%, female-4.04%; mean fecundity-8304.17), followed by September and October 2015. Maturity stage determinatiion showsn that August individuals are classified under late stages of seven-stage maturity scale, while September and October individuals are classified under early stages of the scale. Liver condition of this species also was observed and variations in terms of soze, weight and color of luiver are obtained. Besides, the presence and level of tetrodotoxin (TTX) in four body parts, which are muscle (72.3 MU/g), gonad (167.4 MU/g), liver (184.9 MU/g) and intestine (185.1 (MU/g) were determined by using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).