Effects of yeast extract concentrations on biomass and production of bacteriocin

• Main Author: Khairunnisa, Binti Othman.
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2009
• Subjects: QD Chemistry
• Online Access: http://ir.unimas.my/id/eprint/18756/1/Khairunnisa%20Othman%20ft.pdf; http://ir.unimas.my/id/eprint/18756/

Optimum glucose and yeast extract concentrations for nisin Z production was analyzed on Lactococcus lactis 10-1 where different glucose and yeast extract concentrations were supplemented into the fennentation medium. Glucose with concentrations of 15g11 and 30gll were tested with yeast extract at different concentrations of 10gl1 and 20gll. From the results obtained, a combination of 30gll glucose and 10gll yeast extract showed production of nisin Z that was higher than standard fermentation at 15.25 log AU/ml with specific productivity at 1.24 x 1012 AU/g-DCW. The increased was recorded at 1.28 fold higher than standard fennentation using 30gll glucose and 5g1l yeast extract. The other parameters recorded reductions in nisin Z production as to compare with standard fermentation. The best combination of glucose and yeast extract for nisin Z production is at 30gll and 10gli respectively and clearly, using 10gl1 yeast extract was better than 5g11 and 20gll.