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Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines

Chikungunya is a mosquito-borne disease that has afflicted millions of people worldwide. Infection by the Chikungunya virus (CHIKV) causes symptoms including fever, rashes and arthralgia. The main aim of this study is to compare CHIKV propagated in two cell lines; Vero and C6/36 cell lines to identi...

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Main Author: Ridzwanurahim, bin Nazimudin
Format: Final Year Project Report
Language:English
Published: Universiti Malaysia Sarawak (UNIMAS) 2015
Subjects:
QL Zoology
RB Pathology
Online Access:http://ir.unimas.my/id/eprint/18373/1/Ridwanurahim%20ft.pdf
http://ir.unimas.my/id/eprint/18373/
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spelling my.unimas.ir.183732024-03-13T07:36:39Z http://ir.unimas.my/id/eprint/18373/ Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines Ridzwanurahim, bin Nazimudin QL Zoology RB Pathology Chikungunya is a mosquito-borne disease that has afflicted millions of people worldwide. Infection by the Chikungunya virus (CHIKV) causes symptoms including fever, rashes and arthralgia. The main aim of this study is to compare CHIKV propagated in two cell lines; Vero and C6/36 cell lines to identify the more conducive cell line for downstream assays. This was done by first culturing the cell lines before infecting the cell lines with the virus for viral replication. Cytopathic effects were observed in both cell lines, viral harvest titres were determined through viral plaque assay, and reverse-transcription polymerase chain reaction (RT-PCR) was conducted to confirm CHIKV along with viral RNA quantification. Findings suggest the C6/36 cell line to be the more conducive cell line. Universiti Malaysia Sarawak (UNIMAS) 2015 Final Year Project Report NonPeerReviewed text en http://ir.unimas.my/id/eprint/18373/1/Ridwanurahim%20ft.pdf Ridzwanurahim, bin Nazimudin (2015) Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines. [Final Year Project Report] (Unpublished)
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic QL Zoology
RB Pathology
spellingShingle QL Zoology
RB Pathology
Ridzwanurahim, bin Nazimudin
Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines
description Chikungunya is a mosquito-borne disease that has afflicted millions of people worldwide. Infection by the Chikungunya virus (CHIKV) causes symptoms including fever, rashes and arthralgia. The main aim of this study is to compare CHIKV propagated in two cell lines; Vero and C6/36 cell lines to identify the more conducive cell line for downstream assays. This was done by first culturing the cell lines before infecting the cell lines with the virus for viral replication. Cytopathic effects were observed in both cell lines, viral harvest titres were determined through viral plaque assay, and reverse-transcription polymerase chain reaction (RT-PCR) was conducted to confirm CHIKV along with viral RNA quantification. Findings suggest the C6/36 cell line to be the more conducive cell line.
format Final Year Project Report
author Ridzwanurahim, bin Nazimudin
author_facet Ridzwanurahim, bin Nazimudin
author_sort Ridzwanurahim, bin Nazimudin
title Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines
title_short Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines
title_full Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines
title_fullStr Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines
title_full_unstemmed Comparison of chikungunya virus culture propagated in two cell line; vero and C6/36 cell lines
title_sort comparison of chikungunya virus culture propagated in two cell line; vero and c6/36 cell lines
publisher Universiti Malaysia Sarawak (UNIMAS)
publishDate 2015
url http://ir.unimas.my/id/eprint/18373/1/Ridwanurahim%20ft.pdf
http://ir.unimas.my/id/eprint/18373/
_version_ 1794643993636634624
score 13.214268

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