Astigmatism compensation in digital holographic microscopy using complex-amplitude correlation

• Main Authors: Tamrin, K. F., Rahmatullah, B., Samuri, S. M.
• Format: E-Article
• Published: AIP Publishing 2015
• Subjects: TJ Mechanical engineering and machinery
• Online Access: http://ir.unimas.my/id/eprint/17770/; http://aip.scitation.org/doi/pdf/10.1063/1.4919175

Digital holographic microscopy (DHM) is a promising tool for a three-dimensional imaging of microscopic particles. It offers the possibility of wavefront processing by manipulating amplitude and phase of the recorded digital holograms. With a view to compensate for aberration in the reconstructed particle images, this paper discusses a new approach of aberration compensation based on complex amplitude correlation and the use of a priori information. The approach is applied to holograms of microscopic particles flowing inside a cylindrical micro-channel recorded using an off-axis digital holographic microscope. The approach results in improvements in the image and signal qualities.