Analysis of 12s mitochondriardna genes in the insectivorous bats, hipposideros genus of Sarawak

• Main Author: Puk, Donald Leung Fong.
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2004
• Subjects: QL Zoology
• Online Access: http://ir.unimas.my/id/eprint/17612/1/Analysis%20of%2012s%20mitochondria%20rDNA%20genes%20in%20the%20insectivorous%20bats%2C%20Hipposideros%20genus%20of%20Sarawak%20%2824%20pages%29.pdf; http://ir.unimas.my/id/eprint/17612/8/Puk%20Leung%20Fong.pdf; http://ir.unimas.my/id/eprint/17612/

Individual species within many taxonomic orders encounter difficulties in classification due to variation in observations, and inaccurate morphological measurements. Limited studies have been done to evaluate the molecular data of bats, particularly the Hipposideros genus in Borneo. Polymerase Chain Reaction (PCR) and direct sequencing had been deployed to isolate and analysis partial 12S rONA gene fragment from about 430 bp of the mitochondria genome. Fresh samples were obtained from two caverns in Bau; the Wind Cave and Fairy Cave. Nine species were captured and identified as Hipposideros ater, H. hicolor, H. dyacorum, H. ridleyi, H. cervinus, H. galeritus, H. coxi, H. larvatus and H. diadema. Twenty-three samples were extracted with only one negative result. Fourteen individual samples of various species were successfully amplified by PCR although no sequence data was obtained from them in this study.