Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells
Encephalitis has been described worldwide as a severe complication in patients infected by dengue virus. Reactive oxygen species (ROS) production is a key mechanism involved in the neuronal damage caused by viral encephalitis. In the present study, the capability of dengue virus serotypes 2 (DENV2)...
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National Institute of Infectious Diseases
2017
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my.unimas.ir.171582023-03-31T07:45:54Z http://ir.unimas.my/id/eprint/17158/ Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells Lattapon, Suwanprinya Noppawan, Phumala Morales Pimtip, Sanvarinda Hamady, Dieng Tamaki, Okabayashi Ronald Enrique, Morales Vargas GE Environmental Sciences Encephalitis has been described worldwide as a severe complication in patients infected by dengue virus. Reactive oxygen species (ROS) production is a key mechanism involved in the neuronal damage caused by viral encephalitis. In the present study, the capability of dengue virus serotypes 2 (DENV2) and DENV4 to induce ROS production was investigated in a rat microglial cell line, HAPI cells. The cells were infected with DENV2 and DENV4 at a multiplicity of infection of 0.1 for a 2-h adsorption period. Japanese encephalitis virus (JEV) was used as the reference. DENV2- and DENV4-induced microglial activation and significantly increased ROS production corresponded to decreased cell viability. The activity of DENV4 was significantly higher than the activities of DENV2 and JEV at 48 and 72 h post infection. DENV4 partly induced ROS production via an iron-induced Fenton reaction, as demonstrated by the treatment with an iron chelator, deferiprone. Despite the induction of increased inducible nitric oxide synthase expression and nitric oxide (NO) production by JEV, DENV2, and DENV4 did not induce NO production, suggesting the activation of different pathways in response to infections by different viruses. In conclusion, DENV2 and DENV4 have the capability to induce ROS production and activate microglia, which have been reported as the key components of neuronal damage. National Institute of Infectious Diseases 2017 Article PeerReviewed text en http://ir.unimas.my/id/eprint/17158/1/Dengue.pdf Lattapon, Suwanprinya and Noppawan, Phumala Morales and Pimtip, Sanvarinda and Hamady, Dieng and Tamaki, Okabayashi and Ronald Enrique, Morales Vargas (2017) Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells. Japanese Journal of Infectious Diseases, 70 (4). pp. 383-387. ISSN 1884-2836 https://www.jstage.jst.go.jp/article/yoken/70/4/70_JJID.2016.236/_article DOI: 10.7883/yoken.JJID.2016.236 |
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GE Environmental Sciences Lattapon, Suwanprinya Noppawan, Phumala Morales Pimtip, Sanvarinda Hamady, Dieng Tamaki, Okabayashi Ronald Enrique, Morales Vargas Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells |
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Encephalitis has been described worldwide as a severe complication in patients infected by dengue virus. Reactive oxygen species (ROS) production is a key mechanism involved in the neuronal damage caused by viral encephalitis. In the present study, the capability of dengue virus serotypes 2 (DENV2) and DENV4 to induce ROS production was investigated in a rat microglial cell line, HAPI cells. The cells were infected with DENV2 and DENV4 at a multiplicity of infection of 0.1 for a 2-h adsorption period. Japanese encephalitis virus (JEV) was used as the reference. DENV2- and DENV4-induced microglial activation and significantly increased ROS production corresponded to decreased cell viability. The activity of DENV4 was significantly higher than the activities of DENV2 and JEV at 48 and 72 h post infection. DENV4 partly induced ROS production via an iron-induced Fenton reaction, as demonstrated by the treatment with an iron chelator, deferiprone. Despite the induction of increased inducible nitric oxide synthase expression and nitric oxide (NO) production by JEV, DENV2, and DENV4 did not induce NO production, suggesting the activation of different pathways in response to infections by different viruses. In conclusion, DENV2 and DENV4 have the capability to induce ROS production and activate microglia, which have been reported as the key components of neuronal damage. |
format |
Article |
author |
Lattapon, Suwanprinya Noppawan, Phumala Morales Pimtip, Sanvarinda Hamady, Dieng Tamaki, Okabayashi Ronald Enrique, Morales Vargas |
author_facet |
Lattapon, Suwanprinya Noppawan, Phumala Morales Pimtip, Sanvarinda Hamady, Dieng Tamaki, Okabayashi Ronald Enrique, Morales Vargas |
author_sort |
Lattapon, Suwanprinya |
title |
Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells |
title_short |
Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells |
title_full |
Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells |
title_fullStr |
Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells |
title_full_unstemmed |
Dengue Virus-Induced Reactive Oxygen Species Production in Rat Microglial Cells |
title_sort |
dengue virus-induced reactive oxygen species production in rat microglial cells |
publisher |
National Institute of Infectious Diseases |
publishDate |
2017 |
url |
http://ir.unimas.my/id/eprint/17158/1/Dengue.pdf http://ir.unimas.my/id/eprint/17158/ https://www.jstage.jst.go.jp/article/yoken/70/4/70_JJID.2016.236/_article |
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1762396660649951232 |
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13.188404 |