Quantitative real-time PCR detection of a harmful unarmoured dinoflagellate, Karlodinium australe (Dinophyceae)

• Main Authors: Nyuk, Fong Kon, Lau, Winnie L.S., Kieng, Soon Hii, Ing, Kuo Law, Sing, Tung Teng, Hong, Chang Lim, Takahashi, Kazuya, Gu, Haifeng, Po, Teen Lim, Chui, Pin Leaw
• Format: E-Article
• Language: English
• Published: Blackwell Publishing Ltd 2017
• Subjects: SH Aquaculture. Fisheries. Angling
• Online Access: http://ir.unimas.my/id/eprint/17074/1/Quantitative%20real-time%20PCR%20detection%20of%20a%20harmful%20%28abstract%29.pdf; http://ir.unimas.my/id/eprint/17074/; http://onlinelibrary.wiley.com/doi/10.1111/pre.12186/full

We investigated a harmful algal bloom (HAB) associated with the massive fish kills in Johor Strait, Malaysia, which recurred a year after the first incident in 2014. This incident has urged for the need to have a rapid and precise method in HAB monitoring. In this study, we develop a SYBR green-...