A Genus-and Species-Specific Nested Polymerase Chain Reaction Malaria Detection Assay For Epidemiologic Studies

• Main Authors: Balbir, Singh, Bobogare, Albino, Cox-Singh, J., Snounou, Georges, Mohammad Shukri, Abdullah, Hasan, Abdul Rahman
• Format: Article
• Language: English
• Published: American Society of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene 1999
• Subjects: R Medicine (General)
• Online Access: http://ir.unimas.my/id/eprint/15838/1/A%20GENUS.pdf; http://ir.unimas.my/id/eprint/15838/; https://www.researchgate.net/publication/12951123_A_Genus-_and_species-specific_nested_polymerase_chain_reaction_malaria_detection_assay_for_epidemiologic_studies

A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum–...