Expression, purification and characterization of meta-cleavage enzyme CarBaBb froim Novosphiongobium sp. K

• Main Authors: Zulkharnain, A.B, Maeda, R., Omori, T.
• Format: Article
• Language: English
• Published: Hibiscus Publisher 2013
• Subjects: Q Science (General); QR Microbiology
• Online Access: http://ir.unimas.my/id/eprint/1571/1/zulkharnain.pdf; http://ir.unimas.my/id/eprint/1571/; http://journal.hibiscuspublisher.com/

The meta-cleavage enzyme carbabb of carbazole-degrader novosphiongobium sp. Ka1 werecloned, expressed and purified to homogeneity in escherichia coli strain. The enzyme was cloned with 6x histidine residues attached at the c-terminal of large subunit carbb for purification using affinity chromatogra...