Sequential saccharification and simulationeous fermentation of pineapple waste for bioethanol production
Bioethanol is a renewable source of energy that can be used as an alternative to overcome the depletion of fuel for transportation. It is also friendly to the environment as the burning of bioethanol emits zero net carbon output to the atmosphere. In this study, pineapple wastes were used to produ...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Year Project Report |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2016
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15467/3/Nur%20Hidayah.pdf http://ir.unimas.my/id/eprint/15467/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Bioethanol is a renewable source of energy that can be used as an alternative to overcome the depletion of
fuel for transportation. It is also friendly to the environment as the burning of bioethanol emits zero net
carbon output to the atmosphere. In this study, pineapple wastes were used to produce bioethanol via
Sequential Saccharification and Simultaneous Fermentation (SSSF) a modified version of Simultaneous
Saccharification and Fermentation (SSF) by using cellulase and Saccharomyces cerevisiae. SSSF was
performed on the pineapple waste at 5.0% (w/v) substrate load, with and without the presence of cellulase,
for five days under anaerobic conditions to compare the bioethanol yield. Phenol-Sulphuric Total
Carbohydrate assay and High Performance Liquid Chromatography (HPLC) were used to analyze the
samples taken from fermentation broth. Based on the results, SSSF of 5% (w/v) treated pineapple waste gives
higher ethanol yield compared to SSSF of 5% (w/v) untreated pineapple wastes which are 5.85 g/l and 2.54
g/l respectively at 120 hour of fermentation. This study has proven that SSSF of pineapple waste was able to
liberate bioethanol and it can be considered to be used as a substrate for industrial scale bioethanol
production in future. |
|---|