Nutrient and Physiological Requirements for Biomass Production of Pestalotiopsis sp. UMAS P14 and Pseudopestalotiopsis sp. UMAS P2005/2592
Fungi are important decomposers in our ecosystem and are useful in metabolite production, bio-degradation and bio-sorption of different substrates and wastes through their mycelial biomass. Fungal species are known to have different preferences for their growth requirements. Two fungal species Pes...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2016
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/15028/1/LATEEF%20ADEBOLA.pdf http://ir.unimas.my/id/eprint/15028/ |
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| Summary: | Fungi are important decomposers in our ecosystem and are useful in metabolite production, bio-degradation and
bio-sorption of different substrates and wastes through their mycelial biomass. Fungal species are known to have
different preferences for their growth requirements. Two fungal species Pestalotiopsis and Pseudopestalotiopsis
useful in many biotechnological applications were studied. Nutrient and physiological requirements for mycelial biomass production such as carbon and nitrogen, pH, light and temperature were investigated. Both fungal
isolates were grown in liquid basal medium supplemented separately with various carbon and nitrogen source
and incubated under different light, pH and temperature conditions for 15 days. In general, Pestalotiopsis sp.
and Pseudopestalotiopsis sp. showed significant preferences for monosaccharide and disaccharide carbon source as compared to sugar alcohols and polysaccharides, whereas ammonium tartrate was more preferred as a
nitrogen source compared to ammonium nitrate, ammonium sulphate and other ammonium salts. These two
fungal species were able to grow and produce good mycelial biomass (223.33 mg for Pseudopestalotiopsis and
136.67 mg for Pestalotiopsis) at temperature range of 15°C to 30°C and (290.00 mg for Pseudopestalotiopsis
and 256.67 mg for Pestalotiopsis) on media pH of slightly acidic to slightly alkaline. However, they showed no
significant preferences between constant light, total darkness and alternate light conditions. The results from this study will be very useful for the mycelial biomass production of Pestalotiopsis sp. and Pseudopestalotiopsis sp. for their biotechnological applications. |
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