In Vitro Propagation of Globba brachyanthera K. Schum
An in vitro propagation system was developed for Globba brachyanthera K.Schum., a potential ornamental plant by surface sterilized adventitious bulbils in 20% Clorox for 20 minutes and cultured on Gamborg B5 medium supplemented with a biocide Plant Preservative Mixture (PPM) and antibiotic tetracycl...
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Malaysian Society for Molecular Biology & Biotechnology
2010
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Online Access: | http://ir.unimas.my/id/eprint/14391/1/In%20Vitro%20Propagation%20of%20Globba%20brachyanthera%20K.Schum%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/14391/ http://www.msmbb.org.my/apjmbb/html181/181ab.pdf |
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my.unimas.ir.143912016-11-22T04:40:33Z http://ir.unimas.my/id/eprint/14391/ In Vitro Propagation of Globba brachyanthera K. Schum Kho, P.E. Hamsawi, Sani Boyce, P.C Lim, S.L S Agriculture (General) An in vitro propagation system was developed for Globba brachyanthera K.Schum., a potential ornamental plant by surface sterilized adventitious bulbils in 20% Clorox for 20 minutes and cultured on Gamborg B5 medium supplemented with a biocide Plant Preservative Mixture (PPM) and antibiotic tetracycline. Shoot tips (2-5mm) obtained from in vitro cultured plantlets were induced to form shoots on Gamborg B5 medium containing 20% sucrose and 2.8 g/L Gelrite supplemented with various concentrations of 6-benzylaminopurine (BAP) ranging from 1.0 to 3.0mg/L, either individually or in combination with alpha-naphthalene acetic acid (NAA) at 0.1 mg/L or 0.5 mg/L. All treatments induced formation of multiple shoots as well as rooting after 8 weeks of culture. The highest multiplication rate of 6.6 shoots per explants was obtained in Gamborg B5 medium supplemented with 3.0 mg/L BAP. The generated shoots elongated on Gamborg B5 medium and the multiplication rate did not change further in all of the successive subcultures. Malaysian Society for Molecular Biology & Biotechnology 2010 E-Article PeerReviewed text en http://ir.unimas.my/id/eprint/14391/1/In%20Vitro%20Propagation%20of%20Globba%20brachyanthera%20K.Schum%20%28abstract%29.pdf Kho, P.E. and Hamsawi, Sani and Boyce, P.C and Lim, S.L (2010) In Vitro Propagation of Globba brachyanthera K. Schum. Asia-Pacific Journal of Molecular Biology and Biotechnology, 18 (1). pp. 19-22. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html181/181ab.pdf |
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S Agriculture (General) Kho, P.E. Hamsawi, Sani Boyce, P.C Lim, S.L In Vitro Propagation of Globba brachyanthera K. Schum |
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An in vitro propagation system was developed for Globba brachyanthera K.Schum., a potential ornamental plant by surface sterilized adventitious bulbils in 20% Clorox for 20 minutes and cultured on Gamborg B5 medium supplemented with a biocide Plant Preservative Mixture (PPM) and antibiotic tetracycline. Shoot tips (2-5mm) obtained from in vitro cultured plantlets were induced to form shoots on Gamborg B5 medium containing 20% sucrose and 2.8 g/L Gelrite supplemented with various concentrations of 6-benzylaminopurine (BAP) ranging from 1.0 to 3.0mg/L, either individually or in combination with alpha-naphthalene acetic acid (NAA) at 0.1 mg/L or 0.5 mg/L. All treatments induced formation of multiple shoots as well as rooting after 8 weeks of culture. The highest multiplication rate of 6.6 shoots per explants was obtained in Gamborg B5 medium supplemented with 3.0 mg/L BAP. The generated shoots elongated on Gamborg B5 medium and the multiplication rate did not change further in all of the successive subcultures. |
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E-Article |
author |
Kho, P.E. Hamsawi, Sani Boyce, P.C Lim, S.L |
author_facet |
Kho, P.E. Hamsawi, Sani Boyce, P.C Lim, S.L |
author_sort |
Kho, P.E. |
title |
In Vitro Propagation of Globba brachyanthera K. Schum |
title_short |
In Vitro Propagation of Globba brachyanthera K. Schum |
title_full |
In Vitro Propagation of Globba brachyanthera K. Schum |
title_fullStr |
In Vitro Propagation of Globba brachyanthera K. Schum |
title_full_unstemmed |
In Vitro Propagation of Globba brachyanthera K. Schum |
title_sort |
in vitro propagation of globba brachyanthera k. schum |
publisher |
Malaysian Society for Molecular Biology & Biotechnology |
publishDate |
2010 |
url |
http://ir.unimas.my/id/eprint/14391/1/In%20Vitro%20Propagation%20of%20Globba%20brachyanthera%20K.Schum%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/14391/ http://www.msmbb.org.my/apjmbb/html181/181ab.pdf |
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13.160551 |