Hydrolysis Of Sago Hampas For Production Of Fermentable Sugars

• Main Author: Habibah, Salleh
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak (UNIMAS) 2009
• Subjects: QD Chemistry; QK Botany
• Online Access: http://ir.unimas.my/id/eprint/1391/5/Habibah%20ft.pdf; http://ir.unimas.my/id/eprint/1391/

Enzymatic hydrolysis of sago hampas produced glucose in the range of 5 to 12 gIL. The study was carried in folD' stages which were drying of hampas, pre-treatment, enzymatic hydrolysis and batch fennentation. In the drying method study, three types of drying method were investigated, namely, oven-dried, air-dried and wet. Among these, oven-dried was the best with percentage of total solids at 5.594'10 -8.31%. In the pre-treatment of sago bampas, three types of pre-treatment were used which were steaming, alkaline and acid pre-treatment. Steaming pre-treatment gave the highest recovery percentage of samples at 34% -36.2% followed by alkaline pnHre8tment at 28.60% -30.20% and acid pre-treatment at 28.53% -29.23%. Pre-treated hampas proceed to enzymatic hydrolysis with the concentration of 5% (w/v) sago hampas was dissolved in 0.2M acetate buffer. Initial pH of enzymatic hydrolysis was fixed at 5.0, temperature 60°C and shaking at 100 rpm. Three steps that involved in enzymatic hydrolysis were pre-hydrolysis, liquefaction and saccharification. Concentrations of enzymes used were 10% cellulase, 2% ~galactosidase and I % of Iiquozyme and spirizyme. Control for this experiment was untreated sago bampas. Steaming pre-treated of sago hampas gave the highest yield of glucose (l1.94±O.08 gIL) with enzymatic convertibility of cellulose was 53.73% The highest yield of glucose (11.94±O.08 gIL) proceed to 24 hours batch fermentation with total working volume was 100 mi. Fermentation efficiency obtained was 80.294'10. This proved that fermentable sugar from sago bampas could be fermented to produce bioethanol.