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Development of single chain variable fragment antibodies against dengue virus

The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned in...

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Main Author: Leong, Yee Wei.
Format: Thesis
Language:English
Published: Universiti Malaysia Sarawak, (UNIMAS) 2006
Subjects:
RA0421 Public health. Hygiene. Preventive Medicine
Online Access:http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/12719/
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spelling my.unimas.ir.127192023-03-07T08:40:15Z http://ir.unimas.my/id/eprint/12719/ Development of single chain variable fragment antibodies against dengue virus Leong, Yee Wei. RA0421 Public health. Hygiene. Preventive Medicine The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens. Universiti Malaysia Sarawak, (UNIMAS) 2006 Thesis NonPeerReviewed text en http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf Leong, Yee Wei. (2006) Development of single chain variable fragment antibodies against dengue virus. Masters thesis, Institute of Health andCommunity Medicine.
institution Universiti Malaysia Sarawak
building Centre for Academic Information Services (CAIS)
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sarawak
content_source UNIMAS Institutional Repository
url_provider http://ir.unimas.my/
language English
topic RA0421 Public health. Hygiene. Preventive Medicine
spellingShingle RA0421 Public health. Hygiene. Preventive Medicine
Leong, Yee Wei.
Development of single chain variable fragment antibodies against dengue virus
description The heavy-chain and light-chain variable region genes of two anti-dengue hybridoma cell line (4G2 and MV8/1/B4-1) were isolated by polymerase chain reaction (PCR) and joined by a flexible peptide linker (Gly4Ser3), as a single-chain variable fragment (scFv) DNA gene. The scFv DNA gene were cloned into a phagemid vector (pCANTAB 5 E) and expressed as a fusion protein with M13 phage in E.coli TG1. Affinity-selection was performed to select and enich scFv phages with strong binding to their respective antigens.
format Thesis
author Leong, Yee Wei.
author_facet Leong, Yee Wei.
author_sort Leong, Yee Wei.
title Development of single chain variable fragment antibodies against dengue virus
title_short Development of single chain variable fragment antibodies against dengue virus
title_full Development of single chain variable fragment antibodies against dengue virus
title_fullStr Development of single chain variable fragment antibodies against dengue virus
title_full_unstemmed Development of single chain variable fragment antibodies against dengue virus
title_sort development of single chain variable fragment antibodies against dengue virus
publisher Universiti Malaysia Sarawak, (UNIMAS)
publishDate 2006
url http://ir.unimas.my/id/eprint/12719/3/Development%20of%20single%20chain%20variable%20fragment%20antibodies%20against%20dengue%20virus-cloning%2C%20expression%20and%20functional%20analysis%20%28fulltext%29.pdf
http://ir.unimas.my/id/eprint/12719/
_version_ 1761623516337143808
score 13.159267

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