Biofilm forming ability and time course study of growth of Salmonella Typhi on fresh produce surfaces
This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber, mango and guava surface, as well as to determine the relationship between time contact and biofilm formation. Crystal violet assay was performed to quantify the biofilm formation based on the value of opti...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
UPM
2012
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/12225/1/Noorlis.pdf http://ir.unimas.my/id/eprint/12225/ http://www.ifrj.upm.edu.my/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | This study aimed to determine the biofilm formation ability by Salmonella Typhi on cucumber,
mango and guava surface, as well as to determine the relationship between time contact and biofilm formation.
Crystal violet assay was performed to quantify the biofilm formation based on the value of optical density at
570 nm of the destaining crystal violet at the specific interval time. The result showed that the attachment of the
bacterial cells on the fresh produce surface increased with the contact time. The readings of OD570 at time 12 h
for cucumber, mango and guava surfaces were 0.824, 0.683 and 0.598, respectively, indicating that the biofilm
formation by Salmonella Typhi on different fresh produce surface varied with time. Since the result showed
that Salmonella Typhi formed biofilm on fresh produce surfaces, hygienic practice from farm to fork including
handling, processing, distribution and storage of the fresh produce should be of concern. |
|---|