The effect of different types of inoculum on different types of substrates on ferulic acid production by ceratocystis paradoxa for biovanillin production

• Main Author: Syed Yaacob, Syariffah Nuratiqah
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
• Subjects: SB Plant culture
• Online Access: http://ir.unimas.my/id/eprint/11054/4/Syed%20Yaacob%28fulltext%29.pdf; http://ir.unimas.my/id/eprint/11054/

This research was done to compare the utilization of pineapple peel and banana peel as substrate for microbial production of ferul ic acid-the precursors of vanillin by Ceratocystis paradoxa under solid state fermentation (SSF). This study was vital as no others research had reported any positive production from Ceratocystis paradoxa as this fungus was known as polyphagous wound parasite causing bJack rot post-harvest disease in pineapple and yet there was no study about solid state fermentation of Ceratocystis paradoxa previously. Apart from that, this researched also aimed to investigate the difference between two different type of inocula; spore suspension and solid mycelium plug used in the fermentation in producing high titre of ferulic acid. From the result, pineapple peel gives the highest ferulic acid production of 5.3885 mg/g compared to banana peel which has the maximum production of only 3.9028 mg/g. Two types of inoculum had no significantly difference since both gives high production of ferulic acid to different substrates. The culture inoculated with mycelium plug of Ceratocystic paradoxa gives high titre of ferulic acid to pineapple peel meanwhile spores suspension to banana peel. Lastly, feruloly esterase activity (FEA) of each culture was also beelTdetermined in this study. Keywords: Ferulic Acid, Pineapple Peel, Banana Peel, Ceratocystis paradoxa, Solid State Fermentation, Feruloly Esterase Activity (FEA), Biovanillin