Lipid accumulation in lipomyces starkeyi MV-8 cultured in glucose media

• Main Author: Khairunnisa, Auma
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
• Subjects: S Agriculture (General); SB Plant culture; SF Animal culture
• Online Access: http://ir.unimas.my/id/eprint/10823/8/Lipid%20Accumulation%20of%20Lipomyces%20Starkeyi%20MV-8%20Cultured%20in%20Glucose%20Media%20%28fulltext%29.pdf; http://ir.unimas.my/id/eprint/10823/

Numerous problems in producing diesel have risen globally. Originally, plant oils and animals fats were used as biodiesel feedstock. However, this feedstock was incompatible due to the competition with food production supplies and large area needed. Hence, oleaginous yeast is looked as a further alternative as biodiesel feedstock. Lipomyces starkeyi is selected as it can accumulate higher lipid than other oleaginous yeasts. This study uses L. starkeyi MV-8 instead wild type strain which regularly produce lesser lipid. The study was performed to determine the lipid production of L. starkeyi MV-8 cultured in 2.5% (w/v) and 5.0% (w/v) concentration of glucose. L. starkeyi MV-8 was propagated for 8 days to determine the highest growth of biomass. Lipid accumulation of L. starkeyi MV-8 was performed at two different concentrations of glucose. Total carbohydrate test and lipid extraction were carried out to find out sugar consumption and lipid production respectively. The highest biomass of L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose were obtained at hour 192 at respective 17.57 g/L and 20.10 g/L, while the highest lipid production of L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose were 4.30 g/L and 6.00 g/L respectively. Based on these results, the percentages of lipid content in the L. starkeyi MV-8 biomass were 24.47% and 29.85% respectively for L. starkeyi MV-8 in 2.5% (w/v) and 5.0% (w/v) of glucose. In this study, L. starkeyi MV-8 cultured in 5.0% (w/v) of glucose concentration gave higher biomass and higher lipid than in 2.5% (w/v) of glucose concentration.