Characterization and Expression Study of Prel2a and Prel2b on early vetebrate development

• Main Author: Jonathan, Chan Chia Jui
• Format: Final Year Project Report
• Language: English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
• Subjects: GE Environmental Sciences; GN Anthropology
• Online Access: http://ir.unimas.my/id/eprint/10772/4/Jonathan%20full.pdf; http://ir.unimas.my/id/eprint/10772/

Proline-rich EVHlligand 1 (Prell) and Proline-rich EVHI ligand 2 (PreI2) belong to Prel gene family. Both genes are homologous and participate in cellular integrin activation. Pre12 interacts with GTPase called Ras in GTP-bound state and is essential in regulating cell adhesion and migration. It has been identified to involve in mechanism assisting endocytosis of epidermal growth factor receptor (EGFR) that participates in development of arious diseases such as cancer. However, the comprehensive and mechanistic model of protein signaling involving cell migration is still unavailable. Hence, Prel2 is selected as candidate gene with zebrafish (Danio rerio) in this study to examine its function. This tudy was done partly to verify the occurrence ofPrel2 gene duplication as well as to fulfill our understanding on the importance of Prel2 during vertebrate development. The methodology involves colony peR, plasmid extraction, DNA sequencing, zebrafish cultivation, RNA probe preparation, in situ hybridization, embryo mounting and microscopy. Through these experiments, Prel2 genes were characterized and plasmid with the gene construct was isolated, purified, and ready for RNA probe synthesis for in situ hybridization of zebrafish.