Detection of bacillus cereus from imported rice in Malaysia
Bacillus cereus is frequently associated with foodborne illness outbreak. The common food vehicles for transmission of B. cereus are rice, rice products and starchy foods. Hence, it is crucial to investigate the biosafety of B. cereus in rice. The bacteria were enumerated by performing Most Probabl...
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| Main Author: | |
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| Format: | Final Year Project Report |
| Language: | English English |
| Published: |
Universiti Malaysia Sarawak, (UNIMAS)
2015
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| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10649/1/Pei%20Shze%20%2824pgs_.pdf http://ir.unimas.my/id/eprint/10649/4/Tan%20Pei%20Shze%20ft.pdf http://ir.unimas.my/id/eprint/10649/ |
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| Summary: | Bacillus cereus is frequently associated with foodborne illness outbreak. The common food vehicles for
transmission of B. cereus are rice, rice products and starchy foods. Hence, it is crucial to investigate the biosafety of B. cereus in rice. The bacteria were enumerated by performing Most Probable Number (MPN) approach
followed by Polymerase Chain Reaction (PCR) Assay targeting gyraseB (gyrB) gene at 475 bp. A total of 20
imported raw rice samples from various countries were evaluated. The finding indicated that the occurrence of B.
cereus was >1100 MPN/g for all the imported raw rice samples. In addition, 100% of the imported raw rice
samples were detected with presence of B. cereus. The result highlights the potential risk of B. cereus to cause
food poisoning in rice. Hence, extra precautions in preparing, handling and storage of the rice are necessary to
prevent microbial contamination. |
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