Titanium Dioxide Nanoparticle-Based Interdigitated Electrodes: A Novel Current to Voltage DNA Biosensor Recognizes E. coli O157:H7
Nanoparticle-mediated bio-sensing promoted the development of novel sensors in the front of medical diagnosis. In the present study, we have generated and examined the potential of titanium dioxide (TiO2) crystalline nanoparticles with aluminium interdigitated electrode biosensor to specifically...
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Main Authors: | , , , , |
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Format: | E-Article |
Language: | English |
Published: |
Plos One Publishing
2015
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Subjects: | |
Online Access: | http://ir.unimas.my/id/eprint/10566/1/NO%20139Titanium%20dioxide%20nanoparticle-based%20interdigitated%20electrodes%20%28abstract%29.pdf http://ir.unimas.my/id/eprint/10566/ http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139766 |
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Summary: | Nanoparticle-mediated bio-sensing promoted the development of novel sensors in the front
of medical diagnosis. In the present study, we have generated and examined the potential
of titanium dioxide (TiO2) crystalline nanoparticles with aluminium interdigitated electrode
biosensor to specifically detect single-stranded E.coli O157:H7 DNA. The performance of
this novel DNA biosensor was measured the electrical current response using a picoammeter.
The sensor surface was chemically functionalized with (3-aminopropyl) triethoxysilane
(APTES) to provide contact between the organic and inorganic surfaces of a singlestranded
DNA probe and TiO2 nanoparticles while maintaining the sensing system’s physical
characteristics. The complement of the target DNA of E. coli O157:H7 to the carboxylate-
probe DNA could be translated into electrical signals and confirmed by the increased
conductivity in the current-to-voltage curves. The specificity experiments indicate that the
biosensor can discriminate between the complementary sequences from the base-mismatched
and the non-complementary sequences. After duplex formation, the complementary
target sequence can be quantified over a wide range with a detection limit of 1.0 x 10-
13M. With target DNA from the lysed E. coli O157:H7, we could attain similar sensitivity. Stability
of DNA immobilized surface was calculated with the relative standard deviation
(4.6%), displayed the retaining with 99% of its original response current until 6 months. This
high-performance interdigitated DNA biosensor with high sensitivity, stability and non-fouling
on a novel sensing platform is suitable for a wide range of biomolecular interactive
analyses. |
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