Transformation of fungal endophyte, ceratocystis paradoxa

• Main Author: Nik Zalikha, binti Nik Mustaffa
• Format: E-LPTA
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/10535/1/Transformation%20of%20Fungal%20Endophyte%2C%20Ceratocystis%20paradoxa%20%2824pgs%29.pdf; http://ir.unimas.my/id/eprint/10535/2/Transformation%20of%20Fungal%20Endophyte%2C%20Ceratocystis%20paradoxa%20%28fulltext%29.pdf; http://ir.unimas.my/id/eprint/10535/

Advancement in modern biotechnology, especially in medicine, food, waste utilization and bioremediation are resulted from exploitation of fungi. Endophyte fungi have a wide range of relationship with host varies from symbiosis, mutualism and pathogenic. The endophyte fungi, Ceratocystis paradoxa is habitually living within wide plant species these includes coconut, pineapple, sugarcane and banana. C. paradoxa was among the amylolytic fungus isolated from sago palm pith. In this research, the Agrobacterium-mediated transformation protocol was used to transform the endophyte fungi, C. paradoxa to carry a binary vector, pGSA1131. The expression of BAR selectable marker genes was shown by the fungi that were able to grow through the top agar supplemented with Basta®. The integration of T-DNA region was determined via polymerase chain reaction (PCR) using GUS primer.