Production and characterization of bioactive metabolites produced by antagonistic bacteria using different types of carbon source

• Main Author: Akmal Athirah, binti Taifor
• Format: Final Year Project Report
• Language: English; English
• Published: Universiti Malaysia Sarawak, (UNIMAS) 2015
• Subjects: Q Science (General)
• Online Access: http://ir.unimas.my/id/eprint/10527/3/Akmal%20Athirah%20binti%20Taifor%2024pgs.pdf; http://ir.unimas.my/id/eprint/10527/6/Akmal%20Athirah%20binti%20Taifor%20ft.pdf; http://ir.unimas.my/id/eprint/10527/

The Gram-positive rhizobacterium, Bacillus amyloliquefaciens have the ability to produce bioactive metabolite to suppress numerous plant fungal diseases. The present study was performed to find out which carbon source, glucose, maltose and sucrose are able to aids the production of bioactive metabolite by the antagonistic bacteria, B. amyloliquefaciens in vitro against three plant pathogenic fungi, Colletotrichum gloeosporioides, Fusarium solani and Phytophthora capsici in dual culture assay. The results showed that after day 7 of dual culture, maltose is showing the highest percentage of inhibition against C. gloeosporioides and P. capsici that are 19.05% and 90.91%, respectively while sucrose has the highest percentage of inhibition against F. solani at 27.14%. However, when the B. amyloliquefaciens with maltose and sucrose was assayed on those enzyme assays such as amylase, cellulose, chitinase and protease, the results showed that B. amyloliquefaciens with sucrose as carbon source had more activity as compared to maltose. Therefore, these shows that sucrose is a potential carbon source for the production of bioactive metabolite of B. amyloliquefaciens against plant pathogenic fungi.