Molecular characterization of Vibrio cholerae O1 and non-O1 from human and environmental sources in Malaysia
A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to an...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cambridge University Press
1999
|
| Subjects: | |
| Online Access: | http://ir.unimas.my/id/eprint/10105/1/Lihan.pdf http://ir.unimas.my/id/eprint/10105/ https://www.researchgate.net/publication/231998329_Molecular_characterization_of_Vibrio_cholerae_O1_and_non-O1_from_human_and_environmental_sources_in_Malaysia |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water)
and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and
1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based
assays, resistance to antibiotics, plasmid pro®les and random ampli®ed polymorphic DNA
(RAPD) analysis. All were resistant to 9 or more of the 17 antibiotics tested. Identical
antibiotic resistance patterns of the isolates may indicate that they share a common mode of
developing antibiotic resistance. Furthermore, the multiple antibiotic resistance indexing
showed that all strains tested originated from high risk contamination. Plasmid pro®le analysis
by agarose gel electrophoresis showed the presence of small plasmids in 12 (7 non-O1 and 5
O1 serotypes) with sizes ranging 1±3±4±6 MDa. The CT gene was detected in all clinical isolates
but was present in only 14 (6 O1 serotype and 8 non-O1 serotype) isolates from environmental
waters. The genetic relatedness of the clinical and environmental Vibrio cholerae O1 and non-
O1 strains was investigated by RAPD ®ngerprinting with four primers. The four primers
generated polymorphisms in all 31 strains of Vibrio cholerae tested, producing bands ranging
from !250 to 4500 bp. The RAPD pro®les revealed a wide variability and no correlation with
the source of isolation. This study provides evidence that Vibrio cholerae O1 and non-O1 have
signi®cant public health implications. |
|---|