Cover Image

Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment

Master of Science in Biomedical Electronic Engineering

Saved in:
Bibliographic Details
Main Author: Nor Aimi, Saad
Other Authors: Siti Khadijah, Zaa'ba, Dr.
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2017
Subjects:
Quartz crystal microbalances
Quartz crystals
Piezoelectric devices
Oscillators, Crystal
Biosensors
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/78041
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.unimap-78041
record_format dspace
spelling my.unimap-780412023-03-07T02:33:16Z Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment Nor Aimi, Saad Siti Khadijah, Zaa'ba, Dr. Quartz crystal microbalances Quartz crystals Piezoelectric devices Oscillators, Crystal Biosensors Master of Science in Biomedical Electronic Engineering This thesis presents a study of frequency response for detection of inactive bacteria using Quartz Crystal Microbalance sensor. The characteristics of frequency response towards detection of bacteria were discussed. The Escherichia coli bacteria with concentration x108 colony forming unit per milliliter were inactivated using new sterilization device which is atmospheric pressure plasma jet. A sensor device was constructed to monitor frequency response of bacteria in different condition by using 9 MHz quartz crystal. For detecting the bacteria sample, the sensor surface was prepared using Self-Assemble Monolayer technique together with Escherichia coli antibody as bioreceptor for antigen recognition. Quartz Crystal Microbalance sensor with gold electrodes were mounted in oscillator circuit and exposed to room temperature. The Transistor-transistor Logic oscillator circuit was utilized since it was able to drive the Quartz Crystal Microbalance sensor in air and liquid. Only one side of the crystal were exposed to the liquid sample by using static measurement. The measured of frequency response were observed in order to investigated the interfacial behavior of bacteria Escherichia coli on surface of sensor. The results show that the bacteria after inactivation have higher frequency shifting than bacteria before plasma treatment. The difference in frequency responses showed that bacteria inactivated by atmospheric plasma device have increment of mass. These situations are caused by several factors such as unspecific molecule presence inside inactive bacteria sample, types of bioreceptor, surface roughness and condition of electrode surface. The analysis also includes piezoelectric effects, experimental conditions and the characteristic of surface roughness. From average result of 10 sensors device utilized in the experiment, 8 were successful in bacteria detection. The detection also depends on condition of electrode surface. Moreover, this system has the ability to detect bacteria active and inactive in liquid situation. 2017 2023-03-07T02:33:16Z 2023-03-07T02:33:16Z Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/78041 en Universiti Malaysia Perlis (UniMAP) Universiti Malaysia Perlis (UniMAP) School of Mechatronic Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Quartz crystal microbalances
Quartz crystals
Piezoelectric devices
Oscillators, Crystal
Biosensors
spellingShingle Quartz crystal microbalances
Quartz crystals
Piezoelectric devices
Oscillators, Crystal
Biosensors
Nor Aimi, Saad
Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
description Master of Science in Biomedical Electronic Engineering
author2 Siti Khadijah, Zaa'ba, Dr.
author_facet Siti Khadijah, Zaa'ba, Dr.
Nor Aimi, Saad
format Thesis
author Nor Aimi, Saad
author_sort Nor Aimi, Saad
title Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
title_short Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
title_full Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
title_fullStr Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
title_full_unstemmed Characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
title_sort characterization of quartz crystal microbalance sensor for detection of bacteria inactivation after plasma treatment
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2017
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/78041
_version_ 1772813092765302784
score 13.222552

Similar Items