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Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor

Master of Science in Nanoelectronic Engineering

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Bibliographic Details
Main Author: Hashwan, Saeed Salem Saeed Ba
Other Authors: Ruslinda, A.Rahim, Dr.
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2017
Subjects:
Biosensors
DNA
Graphene
Carbon nanotubes
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77997
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spelling my.unimap-779972023-03-06T04:10:18Z Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor Hashwan, Saeed Salem Saeed Ba Ruslinda, A.Rahim, Dr. Biosensors DNA Graphene Carbon nanotubes Master of Science in Nanoelectronic Engineering This thesis presents a novel thin film of reduced graphene oxide-multiwalled carbon nanotubes (rGO-MWCNTs) composites as a sensing film electrode for Deoxyribonucleic acid (DNA) immobilization and hybridization detection. This project consisted of three parts, which are the rGO-MWCNTs composite thin film preparation and characterization, the device fabrication processes description, and followed by the DNA immobilization and hybridization. In the first part, the thesis describes the graphene oxide preparation from graphite powder using improved Hummers’ method. Whereas, the multiwalled carbon nanotubes (MWCNTs) was functionalized through nitric acid oxidation process. Chemical reduction process was used to obtain the reduced graphene oxide using hydrazine as reduced agent. The MWCNTs, GO, and rGO-MWCNTs materials were mechanically sprayed on the silicon dioxide (SiO2) surface of the device channel using spray technique. Chitosan solution was mixed with the materials and sprayed on the device surface in order to increase the viscosity of the materials and strengthen their adhesion with the silicon dioxide surface by changing the surface characteristic from hydrophobic to hydrophilic. The morphology of the rGO-MWCNTs composite thin films were observed by field emission scanning electron microscope. The bonding of the rGOMWCNTs were examined using Fourier transform infrared spectroscopy. The phase structure of the materials were confirmed via X-ray powder diffraction. Secondly, the design, fabrication and evaluation of the device were descripted in details. In addition, the device fabrication processes contained of oxidation process for silicon dioxide layer growing, physical vapor deposition process which was used to deposit an aluminum layer on the silicon substrate to form the source and drain, mask designed, printed, and utilized in the pattern transfer process, and photolithography process which was carried out to create the channel of the device. The operation of the electrode is based on the surface charge adsorption of the film material interface. Finally, in the DNA immobilization and hybridization section where the novelty of the research introduced, the biosensor demonstrated high sensitivity to the complementary DNA target with a linear range from 500 pM to 100 pM. Furthermore, the biosensor demonstrated good selectivity, reproducibility, and long-term stability for DNA detection. The device has shown sufficient capability to distinguish between targets complementary DNA and different DNA sequences, such as non-complementary and single-mismatched DNA. The hybridization process of the non-complementary DNA has the smallest response (39 μA) due to the double standard DNA was not effectively formed. Whereas, the singlemismatched DNA has shown less response (55 μA) comparing with the complementary DNA (65 μA) due to the single mismatched base. The device accuracy was investigated and found to be 11.28 %. Since, the biosensor responded very well and demonstrated excellent detection capabilities, it is highly recommended to be used in detecting specific biomarkers and other targeted proteins. 2017 2023-03-06T04:06:11Z 2023-03-06T04:06:11Z Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77997 en Universiti Malaysia Perlis (UniMAP) Universiti Malaysia Perlis (UniMAP) Institute of Nano Electronic Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Biosensors
DNA
Graphene
Carbon nanotubes
spellingShingle Biosensors
DNA
Graphene
Carbon nanotubes
Hashwan, Saeed Salem Saeed Ba
Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
description Master of Science in Nanoelectronic Engineering
author2 Ruslinda, A.Rahim, Dr.
author_facet Ruslinda, A.Rahim, Dr.
Hashwan, Saeed Salem Saeed Ba
format Thesis
author Hashwan, Saeed Salem Saeed Ba
author_sort Hashwan, Saeed Salem Saeed Ba
title Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_short Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_full Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_fullStr Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_full_unstemmed Reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for DNA biosensor
title_sort reduced graphene oxide-multi walled carbon nanotubes hybrid material as electrode for dna biosensor
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2017
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77997
_version_ 1772813087612600320
score 13.222552

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