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Computational design and synthesis of molecular imprinted membranes for selective extraction of Quercetin

Master of Science in Materials Engineering

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Bibliographic Details
Main Author: Siti Fatimah, Kamarudin
Other Authors: Mohd Irfan Hatim, Mohamas Dzahir, Dr.
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2017
Subjects:
Quercetin
Bioflavonoids
Molecular imprinting
Quercetin imprinted membranes (QIM)
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77973
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spelling my.unimap-779732023-03-06T00:43:01Z Computational design and synthesis of molecular imprinted membranes for selective extraction of Quercetin Siti Fatimah, Kamarudin Mohd Irfan Hatim, Mohamas Dzahir, Dr. Quercetin Bioflavonoids Molecular imprinting Quercetin imprinted membranes (QIM) Master of Science in Materials Engineering This thesis presents the fabrication of quercetin imprinted membranes (QIM) for selective extraction of quercetin through surface polymerization method. In the development of QIM, selection of functional monomer and the optimum molar ratio of template-monomer were facilitated by the application of molecular modelling through the use of HyperChem software. Three functional monomers were investigated including acrylamide, methacrylic acid and 4-vinylpyridine and the optimization of pre-polymerization complex was conducted at molar ratio of 1:1 to 1:5 for each of the monomers. The results indicated that molar ratio of 1:4, which involving the interaction between quercetin and acylamide could provide potentially favourable pre-polymerization complex prior to the synthesis of QIM. After this step had been established, QIM were developed based on the computational results. In order to deposit the imprinted layer on the surface of membrane, polyvinylidene fluoride (PVDF) membrane was used as the polymer support and then, they were subjected to thermal polymerization process and subsequent removal of template from the polymer matrix. QIM and non-imprinted membranes (NIM) were synthesized to evaluate and characterize the differences with respect to their morphology, chemical functionality as well as their binding behaviour towards quercetin and other components. The confirmation on the presence of imprinted layer on PVDF membrane was done through FTIR, FESEM and AFM analysis. From the results obtained, it can be summarized that imprinted layer composed of recognition sites were successfully formed and distributed evenly on the surface of QIM. For the binding performance evaluation, batch binding, kinetic binding and selectivity tests were conducted. The maximum binding capacity of QIM in batch binding was 25.63 mg/g, which is higher than binding capacity of NIM with the capacity value of 7.47 mg/g. Apart from that, QIM also exhibits higher adsorption rate at the initial stage of adsorption process and the saturation time was achieved after 3-4 hours contact time. Modelling of isotherm and kinetic adsorption showed that QIM has a homogenous surface and followed Elovich kinetic model, respectively. Finally, the selectivity test of QIM was investigated by using sinensetin and rosmarinic acid as competitive components. It demonstrated that QIM showed higher recognition capability towards quercetin compared to sinensetin and rosmarinic acid. 2017 2023-03-06T00:40:46Z 2023-03-06T00:40:46Z Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77973 en Universiti Malaysia Perlis (UniMAP) Universiti Malaysia Perlis (UniMAP) School of Materials Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Quercetin
Bioflavonoids
Molecular imprinting
Quercetin imprinted membranes (QIM)
spellingShingle Quercetin
Bioflavonoids
Molecular imprinting
Quercetin imprinted membranes (QIM)
Siti Fatimah, Kamarudin
Computational design and synthesis of molecular imprinted membranes for selective extraction of Quercetin
description Master of Science in Materials Engineering
author2 Mohd Irfan Hatim, Mohamas Dzahir, Dr.
author_facet Mohd Irfan Hatim, Mohamas Dzahir, Dr.
Siti Fatimah, Kamarudin
format Thesis
author Siti Fatimah, Kamarudin
author_sort Siti Fatimah, Kamarudin
title Computational design and synthesis of molecular imprinted membranes for selective extraction of Quercetin
title_short Computational design and synthesis of molecular imprinted membranes for selective extraction of Quercetin
title_full Computational design and synthesis of molecular imprinted membranes for selective extraction of Quercetin
title_fullStr Computational design and synthesis of molecular imprinted membranes for selective extraction of Quercetin
title_full_unstemmed Computational design and synthesis of molecular imprinted membranes for selective extraction of Quercetin
title_sort computational design and synthesis of molecular imprinted membranes for selective extraction of quercetin
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2017
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77973
_version_ 1772813066049683456
score 13.214268

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