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Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn

Doctor of Philosophy in Bioprocess Engineering

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Bibliographic Details
Main Author: Sharul Aida, Mohd Shayuti
Other Authors: Zarina, Zakaria, Dr.
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2017
Subjects:
Cultivated mushroom
Pleurotus
Lyophilization
Pleurotus pulmonarius
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77869
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spelling my.unimap-778692023-04-04T02:04:57Z Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn Sharul Aida, Mohd Shayuti Zarina, Zakaria, Dr. Cultivated mushroom Pleurotus Lyophilization Pleurotus pulmonarius Doctor of Philosophy in Bioprocess Engineering P. pulmonarius is cultivated by using spawn inoculated with mycelial cultures obtained from spore or tissue culture. However, the application of spore is prone to contamination while tissue culture application may contribute to somaclonal variation. The mushroom grain spawn can be only stored for 30 days. Hence, this study was conducted to improve production and preservation technique for P. pulmonarius spawns by using the combination of induce asexual sporulation and lyophilization techniques respectively. The chlamydospore was induced using various stress media namely DGlucose Soluble Starch, D-Glucose CaCl.2H₂O, D-Glucose NaCl, D-Glucose PEG6000, D-Glucose Glycerol, and D-Glucose Na₂SO₄. Three parameters namely; pH, temperature, and incubation period were selected for optimization of chlamydospore formation. It was found that, D-glucose soluble starch stress medium showed the highest 4.5 x107 chlamydospore production with highest chlamydospore production achieved after 78 hours, at pH 6 and 26˚C of temperature by using 1.5 x 107 inoculum size. Next, the spawn was pre-treated with 20% mixture of trehalose and skimmed milk lyoprotectant and lyophilized by using freeze dryer (Labconco) at -40ºC for 2 to 3 days. The spawn was sealed and stored at room temperature for 1 month. After that, the spawn was treated with 2 hours of rehydration treatment prior to inoculation on PDA medium. The chlamydospore-lyophilized spawn was then compared with the chlamydospore spawn which preserved using cryopreservation, sub-culturing, and 4˚C refrigeration techniques in aspect of growth rate and yield. Growth kinetic analysis found that chlamydospore spawn exhibited higher growth rate; 0.24d-1 compared to tissue culture spawn. 2017 2023-02-16T09:48:23Z 2023-02-16T09:48:23Z Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77869 en Universiti Malaysia Perlis (UniMAP) Universiti Malaysia Perlis (UniMAP) School of Bioprocess Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Cultivated mushroom
Pleurotus
Lyophilization
Pleurotus pulmonarius
spellingShingle Cultivated mushroom
Pleurotus
Lyophilization
Pleurotus pulmonarius
Sharul Aida, Mohd Shayuti
Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
description Doctor of Philosophy in Bioprocess Engineering
author2 Zarina, Zakaria, Dr.
author_facet Zarina, Zakaria, Dr.
Sharul Aida, Mohd Shayuti
format Thesis
author Sharul Aida, Mohd Shayuti
author_sort Sharul Aida, Mohd Shayuti
title Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_short Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_full Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_fullStr Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_full_unstemmed Induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
title_sort induction of chlamydospores in pleurotus pulmonarius (grey oyster mushroom) as inoculum and development of lyophilized spawn
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2017
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/77869
_version_ 1772813064356233216
score 13.222552

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