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Characterization of immunosensor for early detection of Cucumber Mosaic Virus (CMV) detection in chili

Master of Science in Nano electronic Engineering

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Bibliographic Details
Main Author: Shahrul Aizzam, Ariffin
Other Authors: Uda, Hashim, Prof. Dr.
Format: Thesis
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2017
Subjects:
Transducers, Biomedical
Cucumber mosaic virus
Cucurbitaceae
Plant diseases
Electrochemical sensors
Biosensors
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/76626
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spelling my.unimap-766262022-10-26T00:46:01Z Characterization of immunosensor for early detection of Cucumber Mosaic Virus (CMV) detection in chili Shahrul Aizzam, Ariffin Uda, Hashim, Prof. Dr. Transducers, Biomedical Cucumber mosaic virus Cucurbitaceae Plant diseases Electrochemical sensors Biosensors Master of Science in Nano electronic Engineering Cucumber Mosaic Virus (CMV) is one of the major constraints towards cucurbit crops such as cucumbers, zucchinis, pumpkins, papayas and watermelons production, while for non-cucurbit crops such as chilies, tomatoes, spinach, lettuces, celeries, beans, tobaccos and weeds production in South and Southeast Asia. Based on previous studies approximately 5-10 % annual losses of chili yield in Asia was caused by CMV, which accounts for nearly RM 6.05 billion annual loss in chili production worldwide. This loss has given a huge impact especially to the farmers if there are no serious actions taken. Most farmers are using common approach which is visual observation to detect the CMV on their crops despite the visual observation is difficult to identify a symptom caused by the CMV since the visual of symptom depends on the concentration of virus itself and because of that reason, this method is not reliable to eradicate this virus from scratch. Therefore, a portable electrochemical immunosensor based cucumber mosaic virus detection like screen-printed carbon electrode was developed and it can be employed whether in laboratory and field that is essential. In addition, study on the CMV disease and antibody activity was demonstrated to making sensor actively recognize only the CMV molecules by using specify antibody. The CMV purification is used to eliminate the impurities and then, optimization of the purified CMV was performed using sandwich immunoassay format. The purification of antibody was demonstrated to eliminate salt and other proteins and the purified antibody was optimized using sandwich immunoassay format and direct immunoassay format. The initial results showed the both purified substance possess high binding strength. Subsequently, a purified antibody was conjugated with gold nanoparticles and the conjugated solution was used for the immunosensor surface modification to change the immunosensor surface properties. The electrical signal produced from the sensor validation process was measured using chronoamperometric (CM) technique. By using the same technique, the set voltage potential was spotted at 0.2 V and the LOD of immunosensor was at 0.1 mg mL-1. After that, the immunosensor was tested with other pathogens to verify the immunosensor selectivity. The initial study shows the immunosensor fully identifies the purified CMV and did not react to other purified pathogens whereby gives the lowest cross-reactivity. In the CMV screening the same cross-reactivity method was executed and the crude chili leaves that taken around MARDI plantation were used to detect CMV in the chili trees. The results of the CMV screening show the presence of CMV in some samples. Thus, this research provides a sensitive and selective detection tool to the farmers that allow an early detection on their chili plantations. 2017 2022-10-26T00:46:01Z 2022-10-26T00:46:01Z Thesis http://dspace.unimap.edu.my:80/xmlui/handle/123456789/76626 en Universiti Malaysia Perlis (UniMAP) Universiti Malaysia Perlis (UniMAP) Institute of Nano Electronic Engineering
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Transducers, Biomedical
Cucumber mosaic virus
Cucurbitaceae
Plant diseases
Electrochemical sensors
Biosensors
spellingShingle Transducers, Biomedical
Cucumber mosaic virus
Cucurbitaceae
Plant diseases
Electrochemical sensors
Biosensors
Shahrul Aizzam, Ariffin
Characterization of immunosensor for early detection of Cucumber Mosaic Virus (CMV) detection in chili
description Master of Science in Nano electronic Engineering
author2 Uda, Hashim, Prof. Dr.
author_facet Uda, Hashim, Prof. Dr.
Shahrul Aizzam, Ariffin
format Thesis
author Shahrul Aizzam, Ariffin
author_sort Shahrul Aizzam, Ariffin
title Characterization of immunosensor for early detection of Cucumber Mosaic Virus (CMV) detection in chili
title_short Characterization of immunosensor for early detection of Cucumber Mosaic Virus (CMV) detection in chili
title_full Characterization of immunosensor for early detection of Cucumber Mosaic Virus (CMV) detection in chili
title_fullStr Characterization of immunosensor for early detection of Cucumber Mosaic Virus (CMV) detection in chili
title_full_unstemmed Characterization of immunosensor for early detection of Cucumber Mosaic Virus (CMV) detection in chili
title_sort characterization of immunosensor for early detection of cucumber mosaic virus (cmv) detection in chili
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2017
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/76626
_version_ 1751537951958892544
score 13.222552

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