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Production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis

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Main Author: Wong, Soon Thian
Other Authors: Syazni, Zainul Kamal, Dr.
Format: Learning Object
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2021
Subjects:
Hydrolysis
Brown seaweed
Saccharification
Fermentable sugar
Macroalgae
Bioethanol
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73884
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spelling my.unimap-738842022-01-28T02:44:50Z Production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis Wong, Soon Thian Syazni, Zainul Kamal, Dr. School of Bioprocess Engineering Hydrolysis Brown seaweed Saccharification Fermentable sugar Macroalgae Bioethanol Access is limited to UniMAP community. The carbohydrates present in brown seaweed are mainly laminarin and glucans (polysaccharide composed of glucose) such as cellulose. Saccharification is necessary to be performed in order to reduce the crystalinity of cellulose and degrading its intermolecular bonds, thus fermentable sugars is liberated. Saccharification process are carried out by both acid hydrolysis and enzymatic hydrolysis methods and they are complementary. In this research, the parameters that were optimized in acid hydrolysis included the reaction temperature, brown seaweed biomass concentration and sulfuric acid concentration. Meanwhile for enzymatic hydrolysis, the parameters that were optimized included reaction temperature and cellulase enzyme dosage. For both the process, the glucose concentration was analyzed by using Dinitrosalycylic acid (DNS) reagent. The optimization has been carried out by using Central Composite Design (CCD) to analyze the effect of the combination factor toward the response glucose concentration with the aid of Design Expert version 7.1.5 software. The optimum condition for acid hydrolysis were reaction temperature 100.0 C, biomass concentration 3.00 % (w/v) and sulfuric acid concentration 0.10 % (v/v) meanwhile for enzymatic hydrolysis were reaction temperature 45.0 C and enzyme dosage 0.02 mL per 0.30 g biomass. The results shown the highest sugar yield from acid hydrolysis and enzymatic hydrolysis were 3.465 g/L and 6.235 g/L respectively. Under optimized condition, it was examined that both acid and enzymatic would contribute 2.659 g/L and 3.159 g/L respectively to the total glucose yield 5.818 g/L. 2021 2022-01-28T02:44:50Z 2022-01-28T02:44:50Z 2017-06 Learning Object http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73884 en Universiti Malaysia Perlis (UniMAP)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Hydrolysis
Brown seaweed
Saccharification
Fermentable sugar
Macroalgae
Bioethanol
spellingShingle Hydrolysis
Brown seaweed
Saccharification
Fermentable sugar
Macroalgae
Bioethanol
Wong, Soon Thian
Production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis
description Access is limited to UniMAP community.
author2 Syazni, Zainul Kamal, Dr.
author_facet Syazni, Zainul Kamal, Dr.
Wong, Soon Thian
format Learning Object
author Wong, Soon Thian
author_sort Wong, Soon Thian
title Production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis
title_short Production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis
title_full Production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis
title_fullStr Production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis
title_full_unstemmed Production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis
title_sort production of fermentable sugar from brown seaweed with optimization of chemical and enzymatic hydrolysis
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2021
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73884
_version_ 1729704655130198016
score 13.214268

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