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Isolation of biohydrogen producing strain from palm oil sludge pond

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Main Author: Alvin, Ho Zheng Liang
Other Authors: School of Bioprocess Engineering
Format: Other
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2022
Subjects:
Isolation
Biohydrogen
Sludge
Palm oil sludge
Hydrogen
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73352
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spelling my.unimap-733522022-01-13T03:19:22Z Isolation of biohydrogen producing strain from palm oil sludge pond Alvin, Ho Zheng Liang School of Bioprocess Engineering Isolation Biohydrogen Sludge Palm oil sludge Hydrogen Access is limited to UniMAP community. Hydrogen is an environment-friendly source of energy which has the potential to be the alternative fuel to replace the fossil fuels. It is a carbon free fuel which does not provide the greenhouse effect to the environment. Palm oil mill effluent (POME) is an abundant agro-industrial waste in Malaysia which composed of lignocellulosic material. This lignocellulosic biomass can be used as substrate for biogas generation. A biohydrogen producing strain was isolated from submerged palm oil sludge pond. The isolated bacteria was identified by various preliminary test such as Gram stain test, acetone test and gas producing test. The isolated strain was characterized as Gram-positive, rod-shaped and showed a positive result in both acetone test and gas producing test. Tryptone-yeast extract-acetate (TYA) medium was used during biohydrogen production process. The volume of hydrogen produced was measured by water displacement method. The fermentation process was done with the initial glucose concentration of 10 g/L and under anaerobic condition at 37oC. The study resulted in the optimum optical density (OD) of the cell at 1.046 and the residual glucose concentration at 0.2 g/L, where the final pH was at 4.5. The maximum biohydrogen production rate was reached at 46 mL/h when the fermentation period was at 21st hour and it dropped to 33.02 mL/h at 49th hour. Maximum biohydrogen yield was obtained at 292.49 mol-H2/mol-glucose with the highest biogas production and the highest estimation of biohydrogen production was 2,610 mL and 1,618.2 mL respectively. Therefore, this study showed that the locally isolated bacteria had the potential to undergo Acetone-Butanol-Ethanol (ABE) fermentation in order to produce a renewable biofuel. 2022-01-13T03:19:22Z 2022-01-13T03:19:22Z 2017-06 Other http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73352 en Universiti Malaysia Perlis (UniMAP)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Isolation
Biohydrogen
Sludge
Palm oil sludge
Hydrogen
spellingShingle Isolation
Biohydrogen
Sludge
Palm oil sludge
Hydrogen
Alvin, Ho Zheng Liang
Isolation of biohydrogen producing strain from palm oil sludge pond
description Access is limited to UniMAP community.
author2 School of Bioprocess Engineering
author_facet School of Bioprocess Engineering
Alvin, Ho Zheng Liang
format Other
author Alvin, Ho Zheng Liang
author_sort Alvin, Ho Zheng Liang
title Isolation of biohydrogen producing strain from palm oil sludge pond
title_short Isolation of biohydrogen producing strain from palm oil sludge pond
title_full Isolation of biohydrogen producing strain from palm oil sludge pond
title_fullStr Isolation of biohydrogen producing strain from palm oil sludge pond
title_full_unstemmed Isolation of biohydrogen producing strain from palm oil sludge pond
title_sort isolation of biohydrogen producing strain from palm oil sludge pond
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2022
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/73352
_version_ 1724610033215340544
score 13.159267

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