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Efficient conversion of Lignocellulosic biomass to bioethanol by recombinant Saccharomyces Keisuke Cerevisiae

Organized by School of Mechatronic Engineering (UniMAP) & co-organized by The Institution of Engineering Malaysia (IEM), 11th - 13th October 2009 at Batu Feringhi, Penang, Malaysia.

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Main Authors: Abu Saleh, Ahmed, Sinin Hamdan, Syed Tarmizi, Syed Shazali, Makino, Keisuke
Format: Working Paper
Language:English
Published: Universiti Malaysia Perlis 2009
Subjects:
Biomass
Xylose reductase
Saccharomyces cerevisiae
Xylose fermentation
Bioethanol production
Biomass energy
Alcohol as fuel
Lignocellulose > Biotechnology
Online Access:http://dspace.unimap.edu.my/xmlui/handle/123456789/7253
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spelling my.unimap-72532009-11-17T07:00:52Z Efficient conversion of Lignocellulosic biomass to bioethanol by recombinant Saccharomyces Keisuke Cerevisiae Abu Saleh, Ahmed Sinin Hamdan Syed Tarmizi, Syed Shazali Makino, Keisuke Biomass Xylose reductase Saccharomyces cerevisiae Xylose fermentation Bioethanol production Biomass energy Alcohol as fuel Lignocellulose -- Biotechnology Organized by School of Mechatronic Engineering (UniMAP) & co-organized by The Institution of Engineering Malaysia (IEM), 11th - 13th October 2009 at Batu Feringhi, Penang, Malaysia. Today, bioethanol is a promising alternative liquid fuel for gasoline in the transportation sector. However, economically bioethanol produced from lignocellulosics biomass is not competitive with gasoline. Thus, development and optimization for processing techniques are required to realize an extensive fuel ethanol utilization. Xylose is one of the major fermentable sugars present in lignocellulosics biomass, the second most abundant carbohydrate polymer in nature after glucose. The efficient fermentation of xylose is required to develop economically viable processes for producing ethanol from biomass. A recombinant Saccharomyces cerevisiae strain transformed with xylose reductase (XR) and xylitol dehydrogenase (XDH) genes from Pichia stipitis (PsXR and PsXDH, respectively) has the ability to convert xylose to ethanol together with the unfavourable excretion of xylitol, which may be due to intercellular redox imbalance caused by the different coenzyme specificity between NADPH-preferring XR and NAD+-dependent XDH. In this study, we focused on the effect of mutated NADH-preferring PsXR in fermentation. The R276H and K270R/N272D mutants were improved 52-fold and 146-fold, respectively, in the ratio of NADH/NADPH in catalytic efficiency compared with the wild-type (WT). Furthermore, R276H mutation led to significant thermostabilization in PsXR. The most positive effect on xylose fermentation to ethanol was found by using the Y-R276H strain, expressing PsXR R276H mutant and PsXDH WT: 20% increase of ethanol production and 52% decrease of xylitol excretion, compared with the Y-WT strain expressing PsXR WT and PsXDH WT. Measurement of intracellular coenzyme concentrations suggested that maintenance of the of NADPH/NADP+ and NADH/NAD+ ratios is important for efficient ethanol fermentation from xylose by recombinant S. cerevisiae. 2009-11-12T06:52:14Z 2009-11-12T06:52:14Z 2009-10-11 Working Paper p. 3B 1 - 13B 6 978-967-5415-07-4 http://hdl.handle.net/123456789/7253 en Proceedings of International Conference on Applications and Design in Mechanical Engineering 2009 (iCADME 2009) Universiti Malaysia Perlis
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Biomass
Xylose reductase
Saccharomyces cerevisiae
Xylose fermentation
Bioethanol production
Biomass energy
Alcohol as fuel
Lignocellulose -- Biotechnology
spellingShingle Biomass
Xylose reductase
Saccharomyces cerevisiae
Xylose fermentation
Bioethanol production
Biomass energy
Alcohol as fuel
Lignocellulose -- Biotechnology
Abu Saleh, Ahmed
Sinin Hamdan
Syed Tarmizi, Syed Shazali
Makino, Keisuke
Efficient conversion of Lignocellulosic biomass to bioethanol by recombinant Saccharomyces Keisuke Cerevisiae
description Organized by School of Mechatronic Engineering (UniMAP) & co-organized by The Institution of Engineering Malaysia (IEM), 11th - 13th October 2009 at Batu Feringhi, Penang, Malaysia.
format Working Paper
author Abu Saleh, Ahmed
Sinin Hamdan
Syed Tarmizi, Syed Shazali
Makino, Keisuke
author_facet Abu Saleh, Ahmed
Sinin Hamdan
Syed Tarmizi, Syed Shazali
Makino, Keisuke
author_sort Abu Saleh, Ahmed
title Efficient conversion of Lignocellulosic biomass to bioethanol by recombinant Saccharomyces Keisuke Cerevisiae
title_short Efficient conversion of Lignocellulosic biomass to bioethanol by recombinant Saccharomyces Keisuke Cerevisiae
title_full Efficient conversion of Lignocellulosic biomass to bioethanol by recombinant Saccharomyces Keisuke Cerevisiae
title_fullStr Efficient conversion of Lignocellulosic biomass to bioethanol by recombinant Saccharomyces Keisuke Cerevisiae
title_full_unstemmed Efficient conversion of Lignocellulosic biomass to bioethanol by recombinant Saccharomyces Keisuke Cerevisiae
title_sort efficient conversion of lignocellulosic biomass to bioethanol by recombinant saccharomyces keisuke cerevisiae
publisher Universiti Malaysia Perlis
publishDate 2009
url http://dspace.unimap.edu.my/xmlui/handle/123456789/7253
_version_ 1643788770672115712
score 13.214268

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