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Optimization of the isolation of extracellular vesicles via dielectrophoresis: A preliminary analysis

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Main Authors: Nur Mas Ayu, Jamaludin, Muhammad Khairulanwar, Abdul Rahim, Azrul Azlan, Hamzah, Nadiah, Abu, Muhamad Ramdzan, Buyong
Other Authors: muhdramdzan@ukm.edu.my
Format: Article
Language:English
Published: Universiti Malaysia Perlis (UniMAP) 2021
Subjects:
Biomarkers
Dielectrophoresis
Exosomes
Extracellular vesicles
Online Access:http://dspace.unimap.edu.my:80/xmlui/handle/123456789/69810
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spelling my.unimap-698102021-02-23T08:39:33Z Optimization of the isolation of extracellular vesicles via dielectrophoresis: A preliminary analysis Nur Mas Ayu, Jamaludin Muhammad Khairulanwar, Abdul Rahim Azrul Azlan, Hamzah Nadiah, Abu Muhamad Ramdzan, Buyong muhdramdzan@ukm.edu.my Biomarkers Dielectrophoresis Exosomes Extracellular vesicles Link to publisher's homepage at http://ijneam.unimap.edu.my Extracellular vesicles (EVs); 30 nm to 1 micrometer in size; has gained its attention as their role as biomarkers and communicator from one cell to another. The importance of early detection for diseases is highly integral and key to patient's probability of survival. The drawback of many existing methods is that it can be time consuming and prone to falsenegative results due to limitation of the circumstances. This research is aiming to address the disease detection technology limitation by using dielectrophoresis microelectrode principle to manipulate the properties of EVs. The important feature of dielectrophoresis is that this can be used as means to manipulate, transport and separate different types of particles, which one of them is EVs. Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) has been used in this work to characterize the EVs. MATLAB provides the dielectrophoresis technique characterization for manipulation and separation of EVs from their medium. The EVs on the microelectrode has been supplied with 20 V peak-to-peak (Vpp) for frequency in the range of 100 kHz to 10 Mhz to observe the DEP response. Figure 6 shows the movement of EVs at 100 kHz from the region of interest (ROI) to on top of the microelectrode which represents positive dielectrophoresis, PDEP. The experimental testing shows its ability for this method to manipulate the EVs based on their physical characteristics and dielectric properties. Hence, the development of EVs manipulation using dielectrophoresis microelectrode technique offers a faster detection or diagnosis in the medical science field. 2021-02-23T08:39:33Z 2021-02-23T08:39:33Z 2020-12 Article International Journal of Nanoelectronics and Materials, vol.13(Special Issue), 2020, pages 129-142 1985-5761 (Printed) 1997-4434 (Online) http://dspace.unimap.edu.my:80/xmlui/handle/123456789/69810 en NANOSYM, 2019; Universiti Malaysia Perlis (UniMAP)
institution Universiti Malaysia Perlis
building UniMAP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Perlis
content_source UniMAP Library Digital Repository
url_provider http://dspace.unimap.edu.my/
language English
topic Biomarkers
Dielectrophoresis
Exosomes
Extracellular vesicles
spellingShingle Biomarkers
Dielectrophoresis
Exosomes
Extracellular vesicles
Nur Mas Ayu, Jamaludin
Muhammad Khairulanwar, Abdul Rahim
Azrul Azlan, Hamzah
Nadiah, Abu
Muhamad Ramdzan, Buyong
Optimization of the isolation of extracellular vesicles via dielectrophoresis: A preliminary analysis
description Link to publisher's homepage at http://ijneam.unimap.edu.my
author2 muhdramdzan@ukm.edu.my
author_facet muhdramdzan@ukm.edu.my
Nur Mas Ayu, Jamaludin
Muhammad Khairulanwar, Abdul Rahim
Azrul Azlan, Hamzah
Nadiah, Abu
Muhamad Ramdzan, Buyong
format Article
author Nur Mas Ayu, Jamaludin
Muhammad Khairulanwar, Abdul Rahim
Azrul Azlan, Hamzah
Nadiah, Abu
Muhamad Ramdzan, Buyong
author_sort Nur Mas Ayu, Jamaludin
title Optimization of the isolation of extracellular vesicles via dielectrophoresis: A preliminary analysis
title_short Optimization of the isolation of extracellular vesicles via dielectrophoresis: A preliminary analysis
title_full Optimization of the isolation of extracellular vesicles via dielectrophoresis: A preliminary analysis
title_fullStr Optimization of the isolation of extracellular vesicles via dielectrophoresis: A preliminary analysis
title_full_unstemmed Optimization of the isolation of extracellular vesicles via dielectrophoresis: A preliminary analysis
title_sort optimization of the isolation of extracellular vesicles via dielectrophoresis: a preliminary analysis
publisher Universiti Malaysia Perlis (UniMAP)
publishDate 2021
url http://dspace.unimap.edu.my:80/xmlui/handle/123456789/69810
_version_ 1698698670197702656
score 13.214268

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